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Overcoming the inhibitory effects of urea to improve the kinetics of microbial-induced calcium carbonate precipitation (MICCP) by Lysinibacillus sphaericus strain MB284.
Rahmaninezhad, Seyed Ali; Houshmand, Mohammad; Sadighi, Amirreza; Ahmari, Kiana; Kamireddi, Divya; Street, Reva M; Farnam, Yaghoob Amir; Schauer, Caroline L; Najafi, Ahmad Raeisi; Sales, Christopher M.
Afiliación
  • Rahmaninezhad SA; Department of Civil, Architectural, and Environmental Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Houshmand M; Department of Civil, Architectural, and Environmental Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Sadighi A; Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA 19104, USA.
  • Ahmari K; Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Kamireddi D; Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Street RM; Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Farnam YA; Department of Civil, Architectural, and Environmental Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Schauer CL; Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA.
  • Najafi AR; Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, PA 19104, USA.
  • Sales CM; Department of Civil, Architectural, and Environmental Engineering, Drexel University, Philadelphia, PA 19104, USA. Electronic address: chris.sales@drexel.edu.
J Biosci Bioeng ; 138(1): 63-72, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38614831
ABSTRACT
Among different microbial-induced calcium carbonate precipitation (MICCP) mechanisms utilized for biomineralization, ureolysis leads to the greatest yields of calcium carbonate. Unfortunately, it is reported that urea-induced growth inhibition can delay urea hydrolysis but it is not clear how this affects MICCP kinetics. This study investigated the impact of urea addition on the MICCP performance of Lysinibacillus sphaericus MB284 not previously grown on urea (thereafter named bio-agents), compared with those previously cultured in urea-rich media (20 g/L) (hereafter named bio-agents+ or bio-agents-plus). While it was discovered that initial urea concentrations exceeding 3 g/L temporarily hindered cell growth and MICCP reactions for bio-agents, employing bio-agents+ accelerated the initiation of bacterial growth by 33% and led to a 1.46-fold increase in the initial yield of calcium carbonate in media containing 20 g/L of urea. The improved tolerance of bio-agents+ to urea is attributed to the presence of pre-produced endogenous urease, which serves to reduce the initial urea concentration, alleviate growth inhibition, and expedite biomineralization. Notably, elevating the initial concentration of bio-agents+ from OD600 of 0.01 to 1, housing a higher content of endogenous urease, accelerated the initiation of MICCP reactions and boosted the ultimate yield of biomineralization by 2.6 times while the media was supplemented with 20 g/L of urea. These results elucidate the advantages of employing bio-agents+ with higher initial cell concentrations to successfully mitigate the temporary inhibitory effects of urea on biomineralization kinetics, offering a promising strategy for accelerating the production of calcium carbonate for applications like bio self-healing of concrete.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Precipitación Química / Bacillaceae / Urea / Ureasa / Carbonato de Calcio Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Precipitación Química / Bacillaceae / Urea / Ureasa / Carbonato de Calcio Idioma: En Revista: J Biosci Bioeng Asunto de la revista: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Japón