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SOX-10 and TRP-1 expression in feline ocular and nonocular melanomas.
Orlandi, Margherita; Porcellato, Ilaria; Sforna, Monica; Lo Giudice, Adriana; Giglia, Giuseppe; Mechelli, Luca; Brachelente, Chiara.
Afiliación
  • Orlandi M; University of Perugia, Perugia, Italy.
  • Porcellato I; University of Perugia, Perugia, Italy.
  • Sforna M; University of Perugia, Perugia, Italy.
  • Lo Giudice A; University of Perugia, Perugia, Italy.
  • Giglia G; University of Perugia, Perugia, Italy.
  • Mechelli L; University of Perugia, Perugia, Italy.
  • Brachelente C; University of Perugia, Perugia, Italy.
Vet Pathol ; 61(5): 712-720, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38613415
ABSTRACT
In felines, ocular and nonocular melanomas are uncommon tumors that represent a diagnostic challenge for pathologists, especially when amelanotic. To date, the immunohistochemical diagnostic panel in cats is based on specific melanocytic markers (Melan-A and PNL2) and a nonspecific but sensitive marker (S100). In human medicine, SOX-10 is reported to be a sensitive antibody for the detection of melanoma micrometastasis in the lymph node. TRP-1, an enzyme involved in melanogenesis, has recently been used in humans and dogs as a specific melanocyte marker. The aim of this study was to evaluate the cross-reactivity and the expression of SOX-10 and TRP-1 antibodies in feline normal tissue and melanocytic tumors. Thirty-one cases of ocular, cutaneous, and oral melanomas were retrospectively evaluated and confirmed by histopathological examination and by immunolabeling with Melan-A and/or PNL2. SOX-10 nuclear expression in normal tissues was localized in epidermal, subepidermal, hair bulb, and iridal stromal melanocytes and dermal nerves. In melanomas, nuclear expression of SOX-10 was detected in ocular (11/12; 92%), oral (6/7; 86%), and cutaneous sites (12/12; 100%). TRP-1 cytoplasmic immunolabeling in normal tissue was observed in epidermal and bulbar melanocytes and in the lining pigmented epithelium of the iris and in its stroma. Its expression was positively correlated to the degree of pigmentation in the tumor and was observed in 75% of ocular (9/12), 43% of oral (3/7), and 33% of cutaneous melanomas (4/12). This study demonstrated the cross-reactivity of SOX-10 and TRP-1 antibodies in feline non-neoplastic melanocytes and their expression in ocular and nonocular melanomas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Gatos / Neoplasias del Ojo / Factores de Transcripción SOXE / Melanoma Límite: Animals Idioma: En Revista: Vet Pathol Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Gatos / Neoplasias del Ojo / Factores de Transcripción SOXE / Melanoma Límite: Animals Idioma: En Revista: Vet Pathol Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos