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Olive Pomace Extract Contains Low Molecular Weight Peptides and Possesses ACE Inhibitory Activity.
López-Huertas, Eduardo; Rubí-Villegas, Jose; Sánchez-Moreno, Lourdes; Nieto, Rosa.
Afiliación
  • López-Huertas E; Group of Antioxidants and Free Radicals in Biotechnology, Food and Agriculture, Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
  • Rubí-Villegas J; Group of Antioxidants and Free Radicals in Biotechnology, Food and Agriculture, Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
  • Sánchez-Moreno L; Instrumental Technical Services of the Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), Profesor Albareda 1, 18008 Granada, Spain.
  • Nieto R; Department of Nutrition and Sustainable Animal Production, Estación Experimental Zaidín (EEZ), Spanish National Research Council (CSIC), San Miguel 101, 18100 Armilla, Granada, Spain.
Int J Mol Sci ; 25(7)2024 Apr 02.
Article en En | MEDLINE | ID: mdl-38612773
ABSTRACT
The aim of the present study was to determine the ACE inhibitory activity of aqueous extracts of olive pomace and to understand whether they represent a good source of bioactive LMW peptides for nutritional and pharmacological applications. We produced a water extract from olive pomace (var. Picual) and obtained its low molecular weight (LMW) fraction (<3 kDa). The calculated yield of extraction was 100.2 ± 7.9 mg of LMW peptides per 100 g of olive pomace. The olive pomace LMW fraction possessed strong ACE inhibitory activity (IC50 = 3.57 ± 0.22 µg prot/mL). The LMW fraction (<3 kDa) was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. Thirty new peptides, containing between 7-17 amino acids and molecular masses ranging 778-1354 Da, were identified by the Peaks database algorithm using the available Olea europaea (cv. Farga) genome database. Ten new peptides were also identified by Peaks de novo sequencing. The protein sources of twelve peptides detected in the database by Peaks DB were identified by BLAST search. The ACE inhibitory activity of the identified peptides was predicted by BIOPEP software. We conclude that olive pomace possesses ACE inhibitory activity and contains low molecular weight peptides with (predicted) biological activity. Olive pomace may represent a good source of peptides for nutritional and pharmaceutical applications. In our study, it has been shown that olive pomace possesses ACE inhibitory activity and contains low molecular weight peptides with (predicted) biological activity. Olive pomace may represent a good source of peptides for nutritional and pharmaceutical applications. More research is needed in order to identify the in vivo effects of olive pomace bioactive peptides.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Olea Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Olea Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza