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Genetic interference with HvNotch provides new insights into the role of the Notch-signalling pathway for developmental pattern formation in Hydra.
Pan, Qin; Mercker, Moritz; Klimovich, Alexander; Wittlieb, Jörg; Marciniak-Czochra, Anna; Böttger, Angelika.
Afiliación
  • Pan Q; Biocenter, Ludwig-Maximilians-University Munich, Großhaderner Str. 2, 82152, Planegg-Martinsried, Germany. pan@lmu.uni-muenchen.de.
  • Mercker M; Institute of Applied Mathematics, Heidelberg University, Im Neuenheimer Feld 205, 69120, Heidelberg, Germany.
  • Klimovich A; Zoological Institute, Christian-Albrechts-University of Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany.
  • Wittlieb J; Zoological Institute, Christian-Albrechts-University of Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany.
  • Marciniak-Czochra A; Institute of Applied Mathematics, Heidelberg University, Im Neuenheimer Feld 205, 69120, Heidelberg, Germany.
  • Böttger A; Biocenter, Ludwig-Maximilians-University Munich, Großhaderner Str. 2, 82152, Planegg-Martinsried, Germany. boettger@zi.biologie.uni-muenchen.de.
Sci Rep ; 14(1): 8553, 2024 04 12.
Article en En | MEDLINE | ID: mdl-38609434
ABSTRACT
The Notch-signalling pathway plays an important role in pattern formation in Hydra. Using pharmacological Notch inhibitors (DAPT and SAHM1), it has been demonstrated that HvNotch is required for head regeneration and tentacle patterning in Hydra. HvNotch is also involved in establishing the parent-bud boundary and instructing buds to develop feet and detach from the parent. To further investigate the functions of HvNotch, we successfully constructed NICD (HvNotch intracellular domain)-overexpressing and HvNotch-knockdown transgenic Hydra strains. NICD-overexpressing transgenic Hydra showed a pronounced inhibition on the expression of predicted HvNotch-target genes, suggesting a dominant negative effect of ectopic NICD. This resulted in a "Y-shaped" phenotype, which arises from the parent-bud boundary defect seen in polyps treated with DAPT. Additionally, "multiple heads", "two-headed" and "ectopic tentacles" phenotypes were observed. The HvNotch-knockdown transgenic Hydra with reduced expression of HvNotch exhibited similar, but not identical phenotypes, with the addition of a "two feet" phenotype. Furthermore, we observed regeneration defects in both, overexpression and knockdown strains. We integrated these findings into a mathematical model based on long-range gradients of signalling molecules underlying sharply defined positions of HvNotch-signalling cells at the Hydra tentacle and bud boundaries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hydra Límite: Animals Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hydra Límite: Animals Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido