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Direct electrochemical reduction of graphene oxide thin film for aptamer-based selective and highly sensitive detection of matrix metalloproteinase 2.
Jaric, Stefan; Schobesberger, Silvia; Velicki, Lazar; Milovancev, Aleksandra; Nikolic, Stanislava; Ertl, Peter; Bobrinetskiy, Ivan; Knezevic, Nikola Z.
Afiliación
  • Jaric S; Biosense Institute - Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, Dr Zorana Dindica 1, 21000 Novi Sad, Serbia. Electronic address: sjaric@biosense.rs.
  • Schobesberger S; TU Wien, Faculty of Technical Chemistry, Getreidemarkt 9, 1060 Vienna, Austria.
  • Velicki L; Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000, Novi Sad, Serbia; Institute of Cardiovascular Diseases of Vojvodina, Put Doktora Goldmana 4, 21204, Sremska Kamenica, Serbia.
  • Milovancev A; Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000, Novi Sad, Serbia; Institute of Cardiovascular Diseases of Vojvodina, Put Doktora Goldmana 4, 21204, Sremska Kamenica, Serbia.
  • Nikolic S; Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000, Novi Sad, Serbia; Center of Laboratory Medicine, Clinical Center of Vojvodina, Hajduk Veljkova 1, 21000, Novi Sad, Serbia.
  • Ertl P; TU Wien, Faculty of Technical Chemistry, Getreidemarkt 9, 1060 Vienna, Austria.
  • Bobrinetskiy I; Biosense Institute - Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, Dr Zorana Dindica 1, 21000 Novi Sad, Serbia.
  • Knezevic NZ; Biosense Institute - Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, Dr Zorana Dindica 1, 21000 Novi Sad, Serbia. Electronic address: nknezevic@biosense.rs.
Talanta ; 274: 126079, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38608631
ABSTRACT
Simple and low-cost biosensing solutions are suitable for point-of-care applications aiming to overcome the gap between scientific concepts and technological production. To compete with sensitivity and selectivity of golden standards, such as liquid chromatography, the functionalization of biosensors is continuously optimized to enhance the signal and improve their performance, often leading to complex chemical assay development. In this research, the efforts are made on optimizing the methodology for electrochemical reduction of graphene oxide to produce thin film-modified gold electrodes. Under the employed specific conditions, 20 cycles of cyclic voltammetry (CV) are shown to be optimal for superior electrical activation of graphene oxide into electrochemically reduced graphene oxide (ERGO). This platform is further used to develop a matrix metalloproteinase 2 (MMP-2) biosensor, where specific anti-MMP2 aptamers are utilized as a biorecognition element. MMP-2 is a protein which is typically overexpressed in tumor tissues, with important roles in tumor invasion, metastasis as well as in tumor angiogenesis. Based on impedimetric measurements, we were able to detect as low as 3.32 pg mL-1 of MMP-2 in PBS with a dynamic range of 10 pg mL-1 - 10 ng mL-1. Further experiments with real blood samples revealed a promising potential of the developed sensor for direct measurement of MMP-2 in complex media. High specificity of detection is demonstrated - even to the closely related enzyme MMP-9. Finally, the potential of reuse was demonstrated by signal restoration after experimental detection of MMP-2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Metaloproteinasa 2 de la Matriz / Aptámeros de Nucleótidos / Técnicas Electroquímicas / Grafito Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Metaloproteinasa 2 de la Matriz / Aptámeros de Nucleótidos / Técnicas Electroquímicas / Grafito Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos