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Phylogenetic Analysis of Brucella melitensis Strains Isolated from Humans Using 16S rRNA Sequencing and Multiple Locus Variable Number of Tandem Repeats Analysis-16.
Yanmaz, Berna; Özgen, Ediz Kagan; Sayi, Orbay; Erdogan, Yasemin; Aslan, Mehtap Hülya; Iba Yilmaz, Sibel; Karadeniz Pütür, Elif; Polat, Nebahat; Özmen, Murat; Serifoglu Bagatir, Perihan; Ildiz, Sedat.
Afiliación
  • Yanmaz B; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Türkiye.
  • Özgen EK; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Türkiye.
  • Sayi O; Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Izmir, Türkiye.
  • Erdogan Y; Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Erzurum, Türkiye.
  • Aslan MH; Department of Microbiology, Health Sciences University Erzurum Regional Education and Research Hospital, Erzurum, Türkiye.
  • Iba Yilmaz S; Department of Infection Diseases and Clinical Microbiology, Health Sciences University Erzurum Regional Education and Research Hospital, Erzurum, Türkiye.
  • Karadeniz Pütür E; Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Erzurum, Türkiye.
  • Polat N; Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Erzurum, Türkiye.
  • Özmen M; Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Erzurum, Türkiye.
  • Serifoglu Bagatir P; Veterinary Control Institute, Republic of Türkiye Ministry of Agriculture and Forestry, Erzurum, Türkiye.
  • Ildiz S; Graduate School of Health Sciences of Ankara University and General Directorate of Food and Control, Republic of Türkiye Ministry of Agriculture and Forestry, Ankara, Türkiye.
Vector Borne Zoonotic Dis ; 24(7): 416-423, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38608219
ABSTRACT

Background:

Brucellosis is the most important public health problem worldwide, and the annual incidence of the disease in humans is 2.1 million. The Brucella genome is highly conserved, with over 90% similarity among species. The aim of this study was to perform species-level identification of Brucella spp. strains isolated from humans diagnosed with brucellosis and to further investigate the phylogenetic relationships using multiple locus variable number of tandem repeats analysis (MLVA)-16 and 16S rRNA sequencing analysis. Materials and

Methods:

Brucella spp. was isolated from the blood cultures of 54 patients who tested positive for brucellosis through serological examinations. Real-time PCR was used to identify the isolates in species, and the genus level of Brucella was confirmed with 16S rRNA. All isolates were subjected to phylogenetic analysis using variable number of tandem repeat analysis with multiple loci.

Results:

Subsequent analysis via real-time PCR confirmed these isolates to be of the Brucella melitensis species. The 16S rRNA sequence analysis showed 100% homogeneity among the isolates. MLVA revealed the formation of five different genotypic groups. While two groups were formed based on the 16S rRNA sequence analysis, five groups were formed in the MLVA.

Conclusions:

The study concluded that 16S rRNA sequence analysis alone did not provide sufficient discrimination for phylogenetic analysis but served as a supportive method for identification. MLVA exhibited higher phylogenetic power. The widespread isolation of B. melitensis from human brucellosis cases highlights the importance of controlling brucellosis in small ruminants to prevent human infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Brucelosis / ARN Ribosómico 16S / Brucella melitensis / Repeticiones de Minisatélite Límite: Female / Humans / Male Idioma: En Revista: Vector Borne Zoonotic Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Brucelosis / ARN Ribosómico 16S / Brucella melitensis / Repeticiones de Minisatélite Límite: Female / Humans / Male Idioma: En Revista: Vector Borne Zoonotic Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / EPIDEMIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos