Investigations of Limeum Indicum Plant for Diabetes Mellitus and Alzheimer's Disease Dual Therapy: Phytochemical, GC-MS Chemical Profiling, Enzyme Inhibition, Molecular Docking and In-Vivo Studies.
Chem Biodivers
; 21(6): e202301858, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38608202
ABSTRACT
Limeum indicum has been widely utilized in traditional medicine but no experimental work has been done on this herb. The primary objective of this study was to conduct a phytochemical analysis and assess the multifunctional capabilities of aforementioned plant in dual therapy for Alzheimer's disease (AD) and Typeâ
2 diabetes (T2D). The phytochemical screening of ethanol, methanol extract, and their derived fractions of Limeum indicum was conducted using GC-MS, HPLC, UV-analysis and FTIR. The antioxidant capacity was evaluated by DPPH method. The inhibitory potential of the extracts/fractions against α-, ß-glucosidase acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and monoaminine oxidases (MAO-A & B) was evaluated. Results revealed that acetonitrile fraction has highest inhibitory potential against α-glucosidase (IC50=68.47±0.05â
µg/mL), methanol extract against ß-glucosidase (IC50=91.12±0.07â
µg/mL), ethyl acetate fraction against AChE (IC50=59.0±0.02â
µg/mL), ethanol extract against BChE (28.41±0.01â
µg/mL), n-hexane fraction against MAO-A (IC50=150.5±0.31â
µg/mL) and methanol extract for MAO-B (IC50=75.95±0.13â
µg/mL). The docking analysis of extracts\fractions suggested the best binding scores within the active pocket of the respective enzymes. During the in-vivo investigation, ethanol extract produced hypoglycemic effect (134.52±2.79 and 119.38±1.40â
mg/dl) after 21â
days treatment at dose level of 250 and 500â
mg/Kg. Histopathological findings further supported the in-vivo studies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilcolinesterasa
/
Butirilcolinesterasa
/
Extractos Vegetales
/
Enfermedad de Alzheimer
/
Simulación del Acoplamiento Molecular
/
Fitoquímicos
/
Hipoglucemiantes
/
Cromatografía de Gases y Espectrometría de Masas
/
Monoaminooxidasa
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Chem Biodivers
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Pakistán
Pais de publicación:
Suiza