Protocol for the culturing of primary hippocampal mouse neurons for functional in vitro studies.
STAR Protoc
; 5(2): 102991, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38607922
ABSTRACT
Primary hippocampal cultures grown from genetically modified mice provide a simplified context to study molecular mechanisms underlying neuronal development, synaptogenesis, and synapse plasticity in vitro. Here, we describe a simple protocol for culturing hippocampal neurons from P0 to P2 mice and a strategy for inducing alterations in synaptic strength at inhibitory and excitatory synapses in vitro. We also describe approaches for immunofluorescent labeling, image acquisition, and quantification of synaptic proteins. For complete details on the use and execution of this protocol, please refer to Cramer et al.1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hipocampo
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Estados Unidos