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Clinical Value of Different Test Methods in Diagnosing Mycoplasma pneumoniae Infection in Children.
Sun, Xiuling; Yao, Mingzhu; Xu, Peijuan; Jing, Le.
Afiliación
  • Sun X; Department of Pediatrics, Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, China.
  • Yao M; Department of Pediatrics, Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, China.
  • Xu P; Department of Pediatrics, Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, China.
  • Jing L; Department of Pediatrics, Nanjing Tongren Hospital, School of Medicine, Southeast University, Nanjing, China.
Clin Pediatr (Phila) ; : 99228241245343, 2024 Apr 12.
Article en En | MEDLINE | ID: mdl-38606919
ABSTRACT
This research aimed to investigate the diagnostic value of passive particle agglutination test, Mycoplasma pneumoniae (MP) culture, cold agglutination test (CAT), enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction-capillary electrophoresis fragment analysis (PCR-CEFA) for MP infection. Children with respiratory tract infections suspected to be MP infection were subjected to passive particle agglutination test, MP culture, CAT, ELISA, and PCR-CEFA. A total of 146 children (81 males, 65 females, mean age 5.74 ± 3.32 years, and mean course of disease 9.07 ± 5.18 days) met the inclusion criteria. The positivity rate of MP detection by MP culture was 69.18% (101/146). Using the MP culture method as the standard, higher sensitivity and positive predictive value were found in the PCR-CEFA compared with the other 3 methods. Appropriate methods are selected following the advantages and disadvantages of pathogen detection, and pediatric MP infection is analyzed by integrating various test results.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Clin Pediatr (Phila) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Clin Pediatr (Phila) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos