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Decoding the influence of low temperature on biofilm development: The hidden roles of c-di-GMP.
Lin, Yu-Ting; Wang, Yong-Chao; Xue, Yi-Mei; Tong, Zhen; Jiang, Guan-Yu; Hu, Xu-Rui; Crittenden, John C; Wang, Can.
Afiliación
  • Lin YT; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China.
  • Wang YC; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China. Electronic address: wyc@tju.edu.cn.
  • Xue YM; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China.
  • Tong Z; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China.
  • Jiang GY; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China.
  • Hu XR; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China.
  • Crittenden JC; School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.
  • Wang C; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin 300072, China. Electronic address: wangcan@tju.edu.cn.
Sci Total Environ ; 927: 172376, 2024 Jun 01.
Article en En | MEDLINE | ID: mdl-38604376
ABSTRACT
Biofilms are widely used and play important roles in biological processes. Low temperature of wastewater inhibits the development of biofilms derived from wastewater activated sludge. However, the specific mechanism of temperature on biofilm development is still unclear. This study explored the mechanism of temperature on biofilm development and found a feasible method to enhance biofilm development at low temperature. The amount of biofilm development decreased by approximately 66 % and 55 % at 4 °C and 15 °C, respectively, as compared to 28 °C. The cyclic dimeric guanosine monophosphate (c-di-GMP) concentration also decreased at low temperature and was positively correlated with extracellular polymeric substance (EPS) content, formation, and adhesion strength. Microbial community results showed that low temperature inhibited the normal survival of most microorganisms, but promoted the growth of some psychrophile bacteria like Sporosarcina, Caldilineaceae, Gemmataceae, Anaerolineaceae and Acidobacteriota. Further analysis of functional genes demonstrated that the abundance of functional genes related to the synthesis of c-di-GMP (K18968, K18967 and K13590) decreased at low temperature. Subsequently, the addition of exogenous spermidine increased the level of intracellular c-di-GMP and alleviated the inhibition effect of low temperature on biofilm development. Therefore, the possible mechanism of low temperature on biofilm development could be the inhibition of the microorganism activity and reduction of the communication level between cells, which is the closely related to the EPS content, formation, and adhesion strength. The enhancement of c-di-GMP level through the exogenous addition of spermidine provides an alternative strategy to enhance biofilm development at low temperatures. The results of this study enhance the understanding of the influence of temperature on biofilm development and provide possible strategies for enhancing biofilm development at low temperatures.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / GMP Cíclico / Biopelículas Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / GMP Cíclico / Biopelículas Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos