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Novel potential lncRNA biomarker in B cells indicates essential pathogenic pathway activation in patients with SLE.
Zhu, Xinyi; Chen, Yashuo; Yin, Zhihua; Zhang, Yutong; Shen, Yiwei; Dai, Dai; Lin, Xiaojing; Zou, Ling-Hua; Shen, Nan; Ye, Zhizhong; Ding, Huihua; Hou, Guojun.
Afiliación
  • Zhu X; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
  • Chen Y; Shenzhen Futian Hospital for Rheumatic Diseases, Shenzhen, Guangdong, China.
  • Yin Z; Shenzhen Futian Hospital for Rheumatic Diseases, Shenzhen, Guangdong, China.
  • Zhang Y; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
  • Shen Y; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
  • Dai D; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
  • Lin X; Shenzhen Futian Hospital for Rheumatic Diseases, Shenzhen, Guangdong, China.
  • Zou LH; Shenzhen Futian Hospital for Rheumatic Diseases, Shenzhen, Guangdong, China.
  • Shen N; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China.
  • Ye Z; Shenzhen Futian Hospital for Rheumatic Diseases, Shenzhen, Guangdong, China.
  • Ding H; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China houguojun225@163.com dinghuihua@outlook.com.
  • Hou G; Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai, China houguojun225@163.com dinghuihua@outlook.com.
Lupus Sci Med ; 11(1)2024 Apr 10.
Article en En | MEDLINE | ID: mdl-38599668
ABSTRACT

OBJECTIVES:

Systemic lupus erythematosus (SLE) is a highly heterogeneous disease, and B cell abnormalities play a central role in the pathogenesis of SLE. Long non-coding RNAs (lncRNAs) have also been implicated in the pathogenesis of SLE. The expression of lncRNAs is finely regulated and cell-type dependent, so we aimed to identify B cell-expressing lncRNAs as biomarkers for SLE, and to explore their ability to reflect the status of SLE critical pathway and disease activity.

METHODS:

Weighted gene coexpression network analysis (WGCNA) was used to cluster B cell-expressing genes of patients with SLE into different gene modules and relate them to clinical features. Based on the results of WGCNA, candidate lncRNA levels were further explored in public bulk and single-cell RNA-sequencing data. In another independent cohort, the levels of the candidate were detected by RT-qPCR and the correlation with disease activity was analysed.

RESULTS:

WGCNA analysis revealed one gene module significantly correlated with clinical features, which was enriched in type I interferon (IFN) pathway. Among non-coding genes in this module, lncRNA RP11-273G15.2 was differentially expressed in all five subsets of B cells from patients with SLE compared with healthy controls and other autoimmune diseases. RT-qPCR validated that RP11-273G15.2 was highly expressed in SLE B cells and positively correlated with IFN scores (r=0.7329, p<0.0001) and disease activity (r=0.4710, p=0.0005).

CONCLUSION:

RP11-273G15.2 could act as a diagnostic and disease activity monitoring biomarker for SLE, which might have the potential to guide clinical management.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interferón Tipo I / ARN Largo no Codificante / Lupus Eritematoso Sistémico Límite: Humans Idioma: En Revista: Lupus Sci Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interferón Tipo I / ARN Largo no Codificante / Lupus Eritematoso Sistémico Límite: Humans Idioma: En Revista: Lupus Sci Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido