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A dual-signal ratiometric electrochemical aptasensor based on Thi/Au/ZIF-8 and catalytic hairpin assembly for ultra-sensitive detection of aflatoxin B1.
Liu, Jiahui; Wan, Zhigang; Wang, Xinwei; Suo, Zhiguang; Liu, Yong; Wei, Min.
Afiliación
  • Liu J; College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
  • Wan Z; Food Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen, 518045, Guangdong, China.
  • Wang X; College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
  • Suo Z; College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, People's Republic of China.
  • Liu Y; School of Energy Science and Technology, Henan University, Kaifeng, 475004, China.
  • Wei M; College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, People's Republic of China. wei_min80@163.com.
Mikrochim Acta ; 191(5): 256, 2024 04 10.
Article en En | MEDLINE | ID: mdl-38598148
ABSTRACT
A dual-signal ratiometric electrochemical aptasensor has been developed  for AFB1 detection using thionine/Au/zeolitic imidazolate framework-8 (Thi/Au/ZIF-8) nanomaterials and catalytic hairpin assembly (CHA) reaction. Thi/Au/ZIF-8 combined with DNA hairpin 2 (H2) was used as a signal probe. [Fe(CN)6]3-/4- was served as another signal probe, and the IThi/Au/ZIF-8/I[Fe(CN)6]3-/4- ratio was for the first time utilized to quantify AFB1. AFB1-induced CHA was used to expand the ratio of electrical signals. In the presence of AFB1, H2/Thi/Au/ZIF-8 bound to the electrode via CHA, enhanced  the current signal of Thi/Au/ZIF-8. H2 contained the DNA phosphate backbone hindered [Fe(CN)6]3-/4- redox reaction and resulted in a lower [Fe(CN)6]3-/4- current signal. This aptasensor exhibited high specificity for AFB1, a linear range of 0.1 pg mL-1 to 100 ng mL-1, and a detection limit of 0.089 pg mL-1. It demonstrated favorable sensitivity, selectivity, stability, and repeatability. The aptasensor was suitable for detecting AFB1 in peanuts and black tea and holds potential for real sample applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotiazinas / Aflatoxina B1 / Zeolitas Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article Pais de publicación: Austria
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotiazinas / Aflatoxina B1 / Zeolitas Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article Pais de publicación: Austria