Your browser doesn't support javascript.
loading
[Inhibition of glutamatergic neurons in the dorsomedial periaqueductal gray alleviates excessive defensive behaviors of mice with post-traumatic stress disorder].
Li, Y; Guo, Y; Cao, F; Guo, S; Xue, D; Zhou, Z; Hao, X; Tong, L; Fu, Q.
Afiliación
  • Li Y; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
  • Guo Y; Department of Anesthesia, 71st Group Army Hospital of CPLA Army, Xuzhou 221004, China.
  • Cao F; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
  • Guo S; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
  • Xue D; Department of Anesthesia, Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China.
  • Zhou Z; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
  • Hao X; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
  • Tong L; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
  • Fu Q; Department of Anesthesia, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 44(3): 420-427, 2024 Mar 20.
Article en Zh | MEDLINE | ID: mdl-38597432
ABSTRACT

OBJECTIVE:

To investigate the role of glutamatergic neurons in the dorsomedial periaqueductal grey (dmPAG) in regulating excessive defensive behaviors in mice with post-traumatic stress disorder (PTSD).

METHODS:

Eight-week-old male C57BL/6 mice were subjected to stereotactic injections of different recombinant adeno- associated viral vectors (rAAV2/9-CaMKII-mCherry, rAAV2/9-CaMKII-hM3Dq-mCherry and rAAV2/9-CaMKII-hM4Di-mCherry) into the bilateral dmPAG for chemogenetic activation or inhibition of the glutamatergic neurons, followed 2 weeks later by PTSD modeling by single prolonged stress. The looming test, response to whisker stimulation test and contextual fear conditioning (CFC) test were used to observe changes in defensive behaviors of the PTSD mice. The activity of glutamatergic neurons in the dmPAG were observed using immunofluorescence staining.

RESULTS:

Compared with the control mice, the mouse models of PTSD showed a shortened latency of flights with increased time spent in the nest, response scores of defensive behaviors and freezing time (all P<0.01). Immunofluorescence staining revealed significantly increased c-fos-positive glutamatergic neurons in the dmPAG of PTSD mice with defensive behaviors. Activation of the glutamatergic neurons in the dmPAG (in PTSD hM3Dq group) did not cause significant changes in the latency of flights or time in nest but obviously increased response scores of defensive behaviors and freezing time of the mice, whereas inhibiting the glutamatergic neurons in the dmPAG (in PTSD hM4Di group) caused the reverse changes and obviously alleviated defensive behaviors in the PTSD mice (P<0.05 or 0.01).

CONCLUSION:

Inhibiting the activity of glutamatergic neurons in the dmPAG can alleviate defensive behaviors in mice with PTSD.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trastornos por Estrés Postraumático / Sustancia Gris Periacueductal Límite: Animals Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trastornos por Estrés Postraumático / Sustancia Gris Periacueductal Límite: Animals Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China