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Analysis of maternal Fc gamma receptor IIIb isoantibodies using immunomagnetic negative selected neutrophils.
Martins, Juliana Oliveira; Moritz, Elyse; Abbas, Samira Ali; Bayat, Behnaz; Barros, Melca Maria Oliveira; de Marco, Renato; Machado, Renata Fantini; Bordin, José Orlando.
Afiliación
  • Martins JO; Department of Clinical and Experimental Oncology, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Moritz E; Department of Clinical and Experimental Oncology, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Abbas SA; Department of Clinical and Experimental Oncology, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Bayat B; Hospital Geral de Guarulhos, São Paulo, Brazil.
  • Barros MMO; Institute for Clinical Immunology, Transfusion Medicine, and Haemostasis, Justus Liebig University, Giessen, Germany.
  • de Marco R; Department of Clinical and Experimental Oncology, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
  • Machado RF; Instituto de Imunogenética, IGEN, São Paulo, Brazil.
  • Bordin JO; Instituto de Imunogenética, IGEN, São Paulo, Brazil.
Vox Sang ; 119(7): 712-719, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38597364
ABSTRACT
BACKGROUND AND

OBJECTIVES:

The isolation of neutrophils and subsequent detection of anti-human neutrophil antigens (HNA) antibodies are crucial in clinical medicine for the diagnosis of autoimmune neutropenia, neonatal alloimmune neutropenia (NAIN) and transfusion-related acute lung injury (TRALI). This study reports two cases of maternal anti-Fc-gamma-receptor-IIIb (FcγRIIIb) isoimmunization without NAIN symptoms and compares the efficiency of immunomagnetic negative selection (IMNS) with traditional dextran/Ficoll for neutrophil isolation in HNA serological assays. MATERIALS AND

METHODS:

Investigating two cases of maternal anti-FcγRIIIb isoimmunization, neutrophils from three donors were isolated from 8 mL of whole blood using IMNS and dextran/Ficoll. Serological assays included the granulocyte agglutination and immunofluorescence test, monoclonal antibody immobilization of granulocyte antigens and the LABScreen Multi (One Lambda). IMNS and dextran/Ficoll were compared in terms of cell yield, viability, time, cost and purity.

RESULTS:

Maternal anti-FcγRIIIb isoantibodies with FCGR3B gene deletion were detected in both cases. Newborns and fathers exhibited specific gene combinations FCGR3B*02/FCGR3B*02 (Case 1) and FCGR3B*02/FCGR3B*03 (Case 2). IMNS outperformed dextran/Ficoll, yielding four times more neutrophils (average neutrophil counts 18.5 × 103/µL vs. 4.5 × 103/µL), efficiently removing non-neutrophil cells and reducing processing time (30-40 min vs. 70-90 min), although it incurred a higher cost (2.7 times).

CONCLUSION:

Two cases of maternal anti-FcγRIIIb isoantibodies, unrelated to NAIN, were identified. Although neutropenia has not been described in these cases, we emphasize the importance of identifying asymptomatic cases with the potential for severe neutropenia. Additionally, IMNS is introduced as a rapid, high-yield, high-purity neutrophil isolation technique, beneficial for serological assays detecting anti-HNA antibodies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de IgG / Isoanticuerpos / Neutrófilos Límite: Adult / Female / Humans / Male / Newborn / Pregnancy Idioma: En Revista: Vox Sang Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de IgG / Isoanticuerpos / Neutrófilos Límite: Adult / Female / Humans / Male / Newborn / Pregnancy Idioma: En Revista: Vox Sang Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido