Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana: A Telomerase RNA Case Study.
Plants (Basel)
; 13(6)2024 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-38592918
ABSTRACT
The current repertoire of methods available for studying RNA-protein interactions in plants is somewhat limited. Employing an RNA-centric approach, particularly with less abundant RNAs, presents various challenges. Many of the existing methods were initially designed for different model systems, with their application in plants receiving limited attention thus far. The Comprehensive Identification of RNA-Binding Proteins by Mass Spectrometry (ChIRP-MS) technique, initially developed for mammalian cells, has been adapted in this study for application in Arabidopsis thaliana. The procedures have been meticulously modified and optimized for telomerase RNA, a notable example of a low-abundance RNA recently identified. Following these optimization steps, ChIRP-MS can serve as an effective screening method for identifying candidate proteins interacting with any target RNA of interest.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Plants (Basel)
Año:
2024
Tipo del documento:
Article
País de afiliación:
República Checa
Pais de publicación:
Suiza