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Determination of Δ9-tetrahydrocannabinol, 11-nor-carboxy-Δ9-tetrahydrocannabinol and cannabidiol in human plasma and urine after a commercial cannabidiol oil product intake.
Papoutsis, Ioannis; Hatzidouka, Vasiliki; Ntoupa, Stamatina-Panagoula; Angelis, Apostolis; Dona, Artemisia; Sakelliadis, Emmanouil; Spiliopoulou, Chara.
Afiliación
  • Papoutsis I; Department of Forensic Medicine and Toxicology, Medical School, National and Kapodistrian University of Athens, Athens, Greece. ipapoutsis@med.uoa.gr.
  • Hatzidouka V; Department of Forensic Medicine and Toxicology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
  • Ntoupa SP; Division of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
  • Angelis A; Department of Forensic Medicine and Toxicology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
  • Dona A; Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
  • Sakelliadis E; Department of Forensic Medicine and Toxicology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
  • Spiliopoulou C; Department of Forensic Medicine and Toxicology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Forensic Toxicol ; 42(2): 191-201, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38592642
ABSTRACT

PURPOSE:

Cannabidiol (CBD) products are widely used for pain relief, sleep improvement, management of seizures etc. Although the concentrations of Δ9-tetrahydrocannabinol (Δ9-THC) in these products are low (≤0.3% w/w), it is important to investigate if its presence and/or that of its metabolite 11-nor-carboxy-Δ9-THC, is traceable in plasma and urine samples of individuals who take CBD oil products.

METHODS:

A sensitive GC/MS method for the determination of Δ9-THC, 11-nor-carboxy-Δ9-THC and CBD in plasma and urine samples was developed and validated. The sample preparation procedure included protein precipitation for plasma samples and hydrolysis for urine samples, solid-phase extraction and finally derivatization with N,O-bis(trimethylsilyl)trifluoroacetamide) with 1% trimethylchlorosilane.

RESULTS:

For all analytes, the LOD and LOQ were 0.06 and 0.20 ng/mL, respectively. The calibration curves were linear (R2 ≥ 0.992), and absolute recoveries were ≥91.7%. Accuracy and precision were within the accepted range. From the analysis of biologic samples of 10 human participants who were taking CBD oil, it was realized that Δ9-THC was not detected in urine, while 11-nor-carboxy-Δ9-THC (0.69-23.06 ng/mL) and CBD (0.29-96.78 ng/mL) were found in all urine samples. Regarding plasma samples, Δ9-THC (0.21-0.62 ng/mL) was detected in 10, 11-nor-carboxy-Δ9-THC (0.20-2.44 ng/mL) in 35, while CBD (0.20-1.58 ng/mL) in 25 out of 38 samples, respectively.

CONCLUSION:

The results showed that Δ9-THC is likely to be found in plasma although at low concentrations. In addition, the detection of 11-nor-carboxy-Δ9-THC in both urine and plasma samples raises questions and concerns for the proper interpretation of toxicological results, especially considering Greece's zero tolerance law applied in DUID and workplace cases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dronabinol / Cannabidiol / Límite de Detección / Cromatografía de Gases y Espectrometría de Masas Límite: Adult / Humans / Male Idioma: En Revista: Forensic Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dronabinol / Cannabidiol / Límite de Detección / Cromatografía de Gases y Espectrometría de Masas Límite: Adult / Humans / Male Idioma: En Revista: Forensic Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Japón