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NCAPH serves as a prognostic factor and promotes the tumor progression in glioma through PI3K/AKT signaling pathway.
Liang, Jianshen; Yun, Debo; Jin, Wenzhe; Fan, Jikang; Wang, Xuya; Wang, Xisen; Li, Yiming; Yu, Shengping; Zhang, Chen; Li, Tao; Yang, Xuejun.
Afiliación
  • Liang J; Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300000, China.
  • Yun D; Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, 300000, China.
  • Jin W; Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300000, China.
  • Fan J; Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, 300000, China.
  • Wang X; Department of Neurosurgery, Nanchong Central Hospital, Nanchong, 637000, Sichuan, China.
  • Wang X; Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300000, China.
  • Li Y; Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, 300000, China.
  • Yu S; Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding, 071000, Hebei, China.
  • Zhang C; Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300000, China.
  • Li T; Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin, 300000, China.
  • Yang X; Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300000, China.
Mol Cell Biochem ; 2024 Apr 08.
Article en En | MEDLINE | ID: mdl-38587786
ABSTRACT
Non-SMC (Structural Maintenance of Chromosomes) condensin I complex subunit H (NCAPH) has been shown to facilitate progression and predict adverse prognostic outcome in many cancer types. However, the function of NCAPH in gliomas is still unclear. Series of experiments were taken to uncover the function of NCAPH in glioma. The expression of NCAPH and potential mechanism regulating progression of glioma was verified by bioinformatics analysis. Lentiviral transfection was used for establishment of loss-of-function and gain-of-function cell lines. CCK-8 assay and Colony-formation assay were used to evaluate proliferation. Transwell assay and Cell wound healing assay were used to assess migration and invasion. Cell cycle and apoptosis were measured by flow cytometry. Protein and RNA were quantified by WB and RT-PCR, respectively. The nude mice model of glioma was used to evaluate the effect of NCAPH in vivo. The expression of NCAPH increased significantly in glioma tissues and correlated with WHO grade, IDH wild-type and non-1p/19q codeletion. Glioma patients with high expression of NCAPH had an undesirable prognosis. Functionally, upregulated NCAPH promotes the malignant hallmarks of glioma cells in vivo and in vitro. NCAPH correlated with DNA damage repair ability of glioma cells and facilitated the proliferation, invasion, and migration of glioma cells by promoting the PI3K/AKT signaling pathway. This study identifies the important pro-tumor role of NCAPH in glioma and suggests that NCAPH is a potential therapeutic target.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Cell Biochem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Cell Biochem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos