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CD36-mediated podocyte lipotoxicity promotes foot process effacement.
Hua, Wei; Peng, Lan; Chen, Xue-Mei; Jiang, XuShun; Hu, JianGuo; Jiang, Xian-Hong; Xiang, Xu; Wan, Jiangmin; Long, Yingfei; Xiong, Jianqiong; Ma, Xueyi; Du, Xiaogang.
Afiliación
  • Hua W; Department of Nephrology, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing 400000, China.
  • Peng L; Basic Department, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China.
  • Chen XM; Emergency Department, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China.
  • Jiang X; Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China.
  • Hu J; Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
  • Jiang XH; Department of Nephrology, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing 400000, China.
  • Xiang X; Department of Nephrology, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing 400000, China.
  • Wan J; Department of Nephrology, People's Hospital of Qijiang District, Chongqing 401420, China.
  • Long Y; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 401120, China.
  • Xiong J; Hospital of Chongqing University, Chongqing, 400044, China.
  • Ma X; Department of Nephrology, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing 400000, China.
  • Du X; Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
Open Med (Wars) ; 19(1): 20240918, 2024.
Article en En | MEDLINE | ID: mdl-38584832
ABSTRACT

Background:

Lipid metabolism disorders lead to lipotoxicity. The hyperlipidemia-induced early stage of renal injury mainly manifests as podocyte damage. CD36 mediates fatty acid uptake and the subsequent accumulation of toxic lipid metabolites, resulting in podocyte lipotoxicity.

Methods:

Male Sprague-Dawley rats were divided into two groups the normal control group and the high-fat diet group (HFD). Podocytes were cultured and treated with palmitic acid (PA) and sulfo-N-succinimidyl oleate (SSO). Protein expression was measured by immunofluorescence and western blot analysis. Boron-dipyrromethene staining and Oil Red O staining was used to analyze fatty acid accumulation.

Results:

Podocyte foot process (FP) effacement and marked proteinuria occurred in the HFD group. CD36 protein expression was upregulated in the HFD group and in PA-treated podocytes. PA-treated podocytes showed increased fatty acid accumulation, reactive oxygen species (ROS) production, and actin cytoskeleton rearrangement. However, pretreatment with the CD36 inhibitor SSO decreased lipid accumulation and ROS production and alleviated actin cytoskeleton rearrangement in podocytes. The antioxidant N-acetylcysteine suppressed PA-induced podocyte FP effacement and ROS generation.

Conclusions:

CD36 participated in fatty acid-induced FP effacement in podocytes via oxidative stress, and CD36 inhibitors may be helpful for early treatment of kidney injury.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Open Med (Wars) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Open Med (Wars) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia