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Characterization of the fludioxonil and phenamacril dual resistant mutants of Fusarium graminearum.
Wen, Ziyue; Zhang, Yueqi; Chen, Yun; Zhao, Youfu; Shao, Wenyong; Ma, Zhonghua.
Afiliación
  • Wen Z; Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
  • Zhang Y; Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
  • Chen Y; Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China.
  • Zhao Y; Irrigated Agriculture Research and Extension Center, Department of Plant Pathology, Washington State University, Prosser, WA 99350, USA.
  • Shao W; Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China. Electronic address: swy2012102114@163.com.
  • Ma Z; Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, China; State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China.
Pestic Biochem Physiol ; 200: 105815, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38582573
ABSTRACT
Fusarium graminearum is an important fungal pathogen causing Fusarium head blight (FHB) in wheat and other cereal crops worldwide. Due to lack of resistant wheat cultivars, FHB control mainly relies on application of chemical fungicides. Both fludioxonil (a phenylpyrrole compound) and phenamacril (a cyanoacrylate fungicide) have been registered for controlling FHB in China, however, fludioxonil-resistant isolates of F. graminearum have been detected in field. To evaluate the potential risk of dual resistance of F. graminearum to both compounds, fludioxonil and phenamacril dual resistant (DR) mutants of F. graminearum were obtained via fungicide domestication in laboratory. Result showed that resistance of the DR mutants to both fludioxonil and phenamacril were genetically stable after sub-cultured for ten generations or stored at 4 °C for 30 days on fungicide-free PDA. Cross-resistance assay showed that the DR mutants remain sensitive to other groups of fungicides, including carbendazim, tebuconazole, pydiflumetofen, and fluazinam. In addition, the DR mutants exhibited defects in mycelia growth, conidiation, mycotoxin deoxynivalenol (DON) production, and virulence Moreover, the DR mutants displayed increased sensitivity to osmotic stress. Sequencing results showed that amino acid point mutations S217L/T in the myosin I protein is responsible for phenamacril resistance in the DR mutants. Our results indicate that mutations leading to fludioxonil and phenamacril dual resistance could result in fitness cost for F. graminearum. Our results also suggest that the potential risk of F. graminearum developing resistance to both fludioxonil and phenamacril in field could be rather low, which provides scientific guidance in controlling FHB with fludioxonil and phenamacril.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pirroles / Dioxoles / Fungicidas Industriales / Fusarium Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pirroles / Dioxoles / Fungicidas Industriales / Fusarium Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos