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Use of marine occurrent extracts to enhance the stability of dentin extracellular matrix.
Yoo, Hyemin; Gao, Feng; Agostini-Walesch, Gina; Alabsy, Melisa; Mitchell, John C; Carrilho, Marcela R.
Afiliación
  • Yoo H; College of Dental Medicine - Illinois, Midwestern University, Downers Grove, IL, USA.
  • Gao F; College of Dental Medicine - Illinois, Midwestern University, Downers Grove, IL, USA.
  • Agostini-Walesch G; College of Dental Medicine - Arizona, Midwestern University, Glendale, AZ, USA.
  • Alabsy M; College of Dental Medicine - Illinois, Midwestern University, Downers Grove, IL, USA.
  • Mitchell JC; College of Dental Medicine - Arizona, Midwestern University, Glendale, AZ, USA.
  • Carrilho MR; College of Dental Medicine - Illinois, Midwestern University, Downers Grove, IL, USA. Electronic address: marcelacarrilho@gmail.com.
J Mech Behav Biomed Mater ; 154: 106498, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38581962
ABSTRACT
Chitosan (CS) and phloroglucinol (PhG), two extracts abundantly found in marine life, were investigated for their ability to biomodify demineralized dentin by enhancing collagen crosslinks and improving dentin extracellular matrix (ECM) mechanical and biochemical stability. Dentin obtained from non-carious extracted human molars were demineralized with phosphoric acid. Baseline Fourier-transform infrared (FTIR) spectra, apparent flexural elastic modulus (AE) and dry mass (DM) of each specimen were independently acquired. Specimens were randomly incubated for 5 min into either ultrapure water (no-treatment), 1% glutaraldehyde (GA), 1% CS or 1% PhG. Water and GA were used, respectively, as a negative and positive control for collagen crosslinks. Specimens' post-treatment FTIR spectra, AE, and DM were obtained and compared with correspondent baseline measurements. Additionally, the host-derived proteolytic activity of dentin ECM was assessed using hydroxyproline assay (HYP) and spectrofluorometric analysis of a fluorescent-quenched substrate specific for matrix metalloproteinases (MMPs). Finally, the bond strength of an etch-and-rinse adhesive was evaluated after application of marine compounds as non-rinsing dentin primers. Dentin specimens FTIR spectral profile changed remarkably, and their AE increased significantly after treatment with marine compounds. DM variation, HYP assay and fluorogenic substrate analysis concurrently indicated the biodegradation of CS- and PhG-treated specimens was significantly lesser in comparison with untreated specimens. CS and PhG treatments enhanced biomechanical/biochemical stability of demineralized dentin. These novel results show that PhG is a primer with the capacity to biomodify demineralized dentin, hence rendering it less susceptible to biodegradation by host-proteases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recubrimiento Dental Adhesivo / Quitosano Límite: Humans Idioma: En Revista: J Mech Behav Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Recubrimiento Dental Adhesivo / Quitosano Límite: Humans Idioma: En Revista: J Mech Behav Biomed Mater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos