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On-site monitoring of nandrolone in cattle farming samples by portable atmospheric pressure chemical ionization mass spectrometry with ambient sampling.
Wu, Jiahui; Wang, Qingcheng; Tang, Oushan; Liao, Jie; Zhao, Qiaoling; Liang, Jingjing; Feng, Huina; Wang, Linhua; Wang, Haixing; Shen, Qing.
Afiliación
  • Wu J; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.
  • Wang Q; Hangzhou Linping Hospital of Traditional Chinese Medicine, Hangzhou 311199, China.
  • Tang O; Shaoxing Second Hospital, Shaoxing 312000, China.
  • Liao J; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.
  • Zhao Q; Zhoushan Institute of Calibration and Testing for Quality and Technical Supervision, Zhoushan 316021, China.
  • Liang J; Zhejiang Provincial Institute for Food and Drug Control, Hangzhou 310052, China.
  • Feng H; Hangzhou Linping Hospital of Traditional Chinese Medicine, Hangzhou 311199, China.
  • Wang L; Hangzhou Linping Hospital of Traditional Chinese Medicine, Hangzhou 311199, China. Electronic address: wlh001329@sohu.com.
  • Wang H; Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Anti-Drug Laboratory Zhejiang Regional Center, Hangzhou, China. Electronic address: wangdizhu007@163.com.
  • Shen Q; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China. Electronic address: sq@zjgsu.edu.cn.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1238: 124107, 2024 May 01.
Article en En | MEDLINE | ID: mdl-38581929
ABSTRACT
Nandrolone (NT) is a type of androgen anabolic steroid that is often illegally used in cattle farming, leading to unpredictable harm to human health via the food chain. In this study, a rapid detection method for NT in the samples of cattle farming was established using a portable mass spectrometer. The instrument parameters were optimized, including a thermal desorption temperature of 220 °C, a pump speed of 30 %, an APCI ionization voltage of 3900 v, and an injection volume of 6 µL. The samples of bovine urine, feed, sewage, and tissue were selected, and extracted using a solution of methanolacetonitrile (11, v/v), followed by spiking a NT standard solution (1000 ng·mL-1) and ionization through the APCI ion source for detection. The results showed that NT could not be detected in beef and feed due to the complexity of the matrix, while clear signals of NT ions were observed in bovine urine and sewage samples, with LODs of 1000 and 100 ng·mL-1, respectively. Furthermore, quantitative analysis was attempted, and a good linear relationship (R2 = 0.9952) was observed for NT in sewage within the range of 100 to 1000 ng·mL-1. At spiked levels of 100, 500, 1000 and 2000 ng mL-1, the recovery rates ranged from 74.3 % to 92.8 %, with a relative standard deviation (n = 6) of less than 15 %. In conclusion, this detection method offers the advantages of simplicity, rapidity, strong timeliness, and specificity, making it suitable for on-site detection. It can be used for qualitative screening of nandrolone in bovine urine and quantitative analysis of nandrolone in sewage.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Límite de Detección / Nandrolona Límite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Límite de Detección / Nandrolona Límite: Animals Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos