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Innexin hemichannel activation by Microplitis bicoloratus ecSOD monopolymer reduces ROS.
Meng, Jiang-Hui; Huang, Yong-Biao; Long, Jin; Cai, Qiu-Chen; Qiao, Xin; Zhang, Qiong-Li; Zhang, Li-Dan; Yan, Xiang; Jing, Rui; Liu, Xing-Shan; Zhou, Sai-Jun; Yuan, Yong-Sheng; Zhou, Li-Xiang; Peng, Nan-Nan; Li, Xing-Cheng; Cai, Cheng-Hui; Tang, Hong-Mei; Martins, André F; Jiang, Jean X.
Afiliación
  • Meng JH; School of Life Sciences, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Huang YB; Yunnan International Joint Laboratory of Virology & Immunology, Kunming, Yunnan 650500, P.R. China.
  • Long J; Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Cai QC; School of Life Sciences, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Qiao X; Yunnan International Joint Laboratory of Virology & Immunology, Kunming, Yunnan 650500, P.R. China.
  • Zhang QL; Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Zhang LD; School of Life Sciences, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Yan X; Yunnan International Joint Laboratory of Virology & Immunology, Kunming, Yunnan 650500, P.R. China.
  • Jing R; Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Liu XS; Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tübingen, 72076 Tübingen, Germany.
  • Zhou SJ; Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tuebingen, 72076 Tübingen, Germany.
  • Yuan YS; School of Life Sciences, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Yin-Chen Ma; Yunnan International Joint Laboratory of Virology & Immunology, Kunming, Yunnan 650500, P.R. China.
  • Zhou LX; Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Peng NN; School of Life Sciences, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Li XC; Yunnan International Joint Laboratory of Virology & Immunology, Kunming, Yunnan 650500, P.R. China.
  • Cai CH; Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Tang HM; Department of Biochemistry and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78229, USA.
  • Martins AF; School of Life Sciences, Yunnan University, Kunming, Yunnan 650500, P.R. China.
  • Jiang JX; Yunnan International Joint Laboratory of Virology & Immunology, Kunming, Yunnan 650500, P.R. China.
  • Kai-Jun Luo; Key Laboratory of the University in Yunnan Province for International Cooperation in Intercellular Communications and Regulations, Yunnan University, Kunming, Yunnan 650500, P.R. China.
iScience ; 27(4): 109469, 2024 Apr 19.
Article en En | MEDLINE | ID: mdl-38577101
ABSTRACT
The extracellular superoxide dismutases (ecSODs) secreted by Microplitis bicoloratus reduce the reactive oxygen species (ROS) stimulated by the Microplitis bicoloratus bracovirus. Here, we demonstrate that the bacterial transferase hexapeptide (hexapep) motif and bacterial-immunoglobulin-like (BIg-like) domain of ecSODs bind to the cell membrane and transiently open hemichannels, facilitating ROS reductions. RNAi-mediated ecSOD silencing in vivo elevated ROS in host hemocytes, impairing parasitoid larva development. In vitro, the ecSOD-monopolymer needed to be membrane bound to open hemichannels. Furthermore, the hexapep motif in the beta-sandwich of ecSOD49 and ecSOD58, and BIg-like domain in the signal peptides of ecSOD67 were required for cell membrane binding. Hexapep motif and BIg-like domain deletions induced ecSODs loss of adhesion and ROS reduction failure. The hexapep motif and BIg-like domain mediated ecSOD binding via upregulating innexins and stabilizing the opened hemichannels. Our findings reveal a mechanism through which ecSOD reduces ROS, which may aid in developing anti-redox therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos