Your browser doesn't support javascript.
loading
Early morphological and neurochemical changes of the bed nucleus of stria terminalis (BNST) in gestational protein-restricted male offspring.
Torres, D B; Lopes, A; Rodrigues, A J; Ventura-Silva, A P; Sousa, N; Gontijo, J A R; Boer, P A; Lopes, Marcelo Gustavo.
Afiliación
  • Torres DB; Fetal Programming and Hydroelectrolyte Metabolism Laboratory, Internal Medicine Department, School of Medicine, State University of Campinas, Campinas, Brazil.
  • Lopes A; Fetal Programming and Hydroelectrolyte Metabolism Laboratory, Internal Medicine Department, School of Medicine, State University of Campinas, Campinas, Brazil.
  • Rodrigues AJ; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Ventura-Silva AP; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Sousa N; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Gontijo JAR; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Boer PA; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Lopes MG; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Nutr Neurosci ; 27(11): 1250-1268, 2024 Nov.
Article en En | MEDLINE | ID: mdl-38576309
ABSTRACT

BACKGROUND:

The bed nucleus of the stria terminalis (BNST) is a structure with a peculiar neurochemical composition involved in modulating anxietylike behavior and fear.

AIM:

The present study investigated the effects on the BNST neurochemical composition and neuronal structure in critical moments of the postnatal period in gestational protein-restricted male rats' offspring.

METHODS:

Dams were maintained during the pregnancy on isocaloric rodent laboratory chow with standard protein content [NP, 17%] or low protein content [LP, 6%]. BNST from male NP and age-matched LP offspring was studied using the isotropic fractionator method, Neuronal 3D reconstruction, dendritic-tree analysis, blotting analysis, and high-performance liquid chromatography.

RESULTS:

Serum corticosterone levels were higher in male LP offspring than NP rats in 14-day-old offspring, without any difference in 7-day-old progeny. The BNST total cell number and anterodorsal BNST division volume in LP progeny were significantly reduced on the 14th postnatal day compared with NP offspring. The BNST HPLC analysis from 7 days-old LP revealed increased norepinephrine levels compared to NP progeny. The BNST blot analysis from 7-day-old LP revealed reduced levels of GR and BDNF associated with enhanced CRF1 expression compared to NP offspring. 14-day-old LP offspring showed reduced expression of MR and 5HT1A associated with decreased DOPAC and DOPA turnover levels relative to NP rats. In Conclusion, the BNST cellular and neurochemical changes may represent adaptation during development in response to elevated fetal exposure to maternal corticosteroid levels. In this way, gestational malnutrition alters the BNST content and structure and contributes to already-known behavioral changes.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Efectos Tardíos de la Exposición Prenatal / Núcleos Septales / Corticosterona Límite: Animals / Pregnancy Idioma: En Revista: Nutr Neurosci Asunto de la revista: CIENCIAS DA NUTRICAO / NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Efectos Tardíos de la Exposición Prenatal / Núcleos Septales / Corticosterona Límite: Animals / Pregnancy Idioma: En Revista: Nutr Neurosci Asunto de la revista: CIENCIAS DA NUTRICAO / NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido