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Albumen exosomes alleviate LPS-induced inflammation of intestinal epithelial cells via miR-22/ATM/p53/NF-κB axis.
Zhang, Fengdong; Yue, Yanrui; Chen, Jian; Xiao, Pan; Ma, Hui; Feng, Jia; Yang, Mingming; Min, Yuna.
Afiliación
  • Zhang F; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Yue Y; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Chen J; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Xiao P; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Ma H; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • Feng J; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: fengjiacaas@163.com.
  • Yang M; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: ymm@nwsuaf.edu.cn.
  • Min Y; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: minyuna2003@163.com.
Int J Biol Macromol ; 267(Pt 1): 131241, 2024 May.
Article en En | MEDLINE | ID: mdl-38574929
ABSTRACT
Biological macromolecules identified in albumen were found benefit to intestinal health, whether albumen contains exosomes and function of their cargos in intestinal inflammation remain unknown. This study aimed to investigate characteristics and cargos of albumen exosomes, as well as their potential roles in alleviating inflammation in intestinal epithelial cells. Our results demonstrated that albumen contains exosomes that are cup-shaped morphology vesicles with diameter ranging from 50 to 200 nm. There were 278 miRNAs and 45 proteins with higher expression levels in albumen exosomes, and they were mainly involved in immune responses and programmed cell death pathways, including apoptosis and p53 signaling pathway. LPS induced overexpression of pro-inflammatory cytokines IL-1ß and TNF-α and excessive apoptosis, which could be reversed by albumen exosomes. The beneficial effects of exosomes could be mainly attributed to miRNA cargos and their inhibition on inflammatory response signaling pathways (p53 and NF-κB pathways). Mechanically, exosome miR-22 targeted ATM and inhibited p53/NF-κB pathway, alleviating LPS-induced overexpression of Caspase-3 and Bax, and inflammatory response. Collectively, albumen exosomes alleviate inflammation of intestinal epithelial cells via miR-22/ATM/p53/NF-κB axis and these findings may provide theoretical basis to the potential application of albumen exosomes for intestinal inflammation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Lipopolisacáridos / FN-kappa B / Proteína p53 Supresora de Tumor / MicroARNs / Células Epiteliales / Exosomas / Inflamación Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Lipopolisacáridos / FN-kappa B / Proteína p53 Supresora de Tumor / MicroARNs / Células Epiteliales / Exosomas / Inflamación Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos