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Controlled production of a polyhydroxyalkanoate (PHA) tetramer containing different mole fraction of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3 HV), 4 HV and 5 HV units by engineered Cupriavidus necator.
Oh, Suk-Jin; Kim, Suwon; Lee, Yeda; Shin, Yuni; Choi, Suhye; Oh, Jinok; Bhatia, Shashi Kant; Joo, Jeong Chan; Yang, Yung-Hun.
Afiliación
  • Oh SJ; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Kim S; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Lee Y; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Shin Y; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Choi S; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Oh J; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
  • Bhatia SK; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul, Republic of Korea.
  • Joo JC; Department of Chemical Engineering, Kyung Hee University, Kyunggi-do, Republic of Korea.
  • Yang YH; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul, Republic of Korea. Electronic address: seokor@konkuk.ac.kr.
Int J Biol Macromol ; 266(Pt 2): 131332, 2024 May.
Article en En | MEDLINE | ID: mdl-38574905
ABSTRACT
Polyhydroxyalkanoates (PHAs) are promising alternatives to existing petrochemical-based plastics because of their bio-degradable properties. However, the limited structural diversity of PHAs has hindered their application. In this study, high mole-fractions of Poly (39 mol% 3HB-co-17 mol% 3 HV-co-44 mol% 4 HV) and Poly (25 mol% 3HB-co-75 mol% 5 HV) were produced from 4- hydroxyvaleric acid and 5-hydroxyvaleric acid, using Cupriavidus necator PHB-4 harboring the gene phaCBP-M-CPF4 with modified sequences. In addition, the complex toxicity of precursor mixtures was tested, and it was confirmed that the engineered C. necator was capable of synthesizing Poly (32 mol% 3HB-co-11 mol% 3 HV-co-25 mol% 4 HV-co-32 mol% 5 HV) at low mixture concentrations. Correlation analyses of the precursor ratio and the monomeric mole fractions indicated that each mole fractions could be precisely controlled using the precursor proportion. Physical property analysis confirmed that Poly (3HB-co-3 HV-co-4 HV) is a rubber-like amorphous polymer and Poly (3HB-co-5 HV) has a high tensile strength and elongation at break. Poly (3HB-co-3 HV-co-4 HV-co-5 HV) had a much lower glass transition temperature than the co-, terpolymers containing 3 HV, 4 HV and 5 HV. This study expands the range of possible physical properties of PHAs and contributes to the realization of custom PHA production by suggesting a method for producing PHAs with various physical properties through mole-fraction control of 3 HV, 4 HV and 5 HV.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cupriavidus necator / Polihidroxialcanoatos Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cupriavidus necator / Polihidroxialcanoatos Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos