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Biofilm-producing Escherichia coli O104:H4 overcomes bile salts toxicity by expressing virulence and resistance proteins.
Machado, Maxsueli Aparecida Moura; Chapartegui-González, Itziar; Castro, Vinicius Silva; Figueiredo, Eduardo Eustáquio de Souza; Conte-Junior, Carlos Adam; Torres, Alfredo G.
Afiliación
  • Machado MAM; Food Science Program (PPGCAL), Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-909, Brazil.
  • Chapartegui-González I; Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitaria, Rio de Janeiro 21941-598, RJ, Brazil.
  • Castro VS; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, Federal University of Rio de Janeiro (UFRJ), Cidade Universitaria, Rio de Janeiro 21941-909, RJ, Brazil.
  • Figueiredo EES; Department of Microbiology and Immunology, University of Texas Medical Branch (UTMB), Galveston, TX 77555, United States.
  • Conte-Junior CA; Animal Science Program (PPGCA). Federal University of Mato Grosso (UFMT), Cuiabá, Mato Grosso 78060-900, Brazil.
  • Torres AG; Nutrition, Food and Metabolism Program (PPGNAM). Federal University of Mato Grosso (UFMT), Cuiabá, Mato Grosso 78060-900, Brazil.
Lett Appl Microbiol ; 77(4)2024 Apr 08.
Article en En | MEDLINE | ID: mdl-38573831
ABSTRACT
We investigated bile salts' ability to induce phenotypic changes in biofilm production and protein expression of pathogenic Escherichia coli strains. For this purpose, 82 pathogenic E. coli strains isolated from humans (n = 70), and animals (n = 12), were examined for their ability to form biofilms in the presence or absence of bile salts. We also identified bacterial proteins expressed in response to bile salts using sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-electrophoresis) and liquid chromatography-mass spectrometry (LC-MS/MS). Lastly, we evaluated the ability of these strains to adhere to Caco-2 epithelial cells in the presence of bile salts. Regarding biofilm formation, two strains isolated from an outbreak in Republic of Georgia in 2009 were the only ones that showed a high and moderate capacity to form biofilm in the presence of bile salts. Further, we observed that those isolates, when in the presence of bile salts, expressed different proteins identified as outer membrane proteins (i.e. OmpC), and resistance to adverse growth conditions (i.e. F0F1, HN-S, and L7/L12). We also found that these isolates exhibited high adhesion to epithelial cells in the presence of bile salts. Together, these results contribute to the phenotypic characterization of E. coli O104 H4 strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica / Escherichia coli O104 Límite: Animals / Humans Idioma: En Revista: Lett Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica / Escherichia coli O104 Límite: Animals / Humans Idioma: En Revista: Lett Appl Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido