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Monitoring the Mitochondrial Viscosity Changes During Cuproptosis with Iridium(III) Complex Probe via In Situ Phosphorescence Lifetime Imaging.
Yang, Gang-Gang; Zhao, Ying Qing; Zhang, Lan; Sun, Sujuan; Liu, Bin; Han, Xinya.
Afiliación
  • Yang GG; School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui 243002, P. R. China.
  • Zhao YQ; Biochemical Engineering Research Center, Anhui University of Technology, Ma'anshan, Anhui 243002, P. R. China.
  • Zhang L; School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui 243002, P. R. China.
  • Sun S; Biochemical Engineering Research Center, Anhui University of Technology, Ma'anshan, Anhui 243002, P. R. China.
  • Liu B; School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui 243002, P. R. China.
  • Han X; Biochemical Engineering Research Center, Anhui University of Technology, Ma'anshan, Anhui 243002, P. R. China.
Anal Chem ; 96(15): 5931-5939, 2024 04 16.
Article en En | MEDLINE | ID: mdl-38573171
ABSTRACT
Cuproptosis is a novel copper-dependent form of programmed cell death, displaying important regulatory functions in many human diseases, including cancer. However, the relationship between the changes in mitochondrial viscosity, a key factor associated with cellular malfunction, and cuproptosis is still unclear. Herein, we prepared a phosphorescent iridium (Ir) complex probe for precisely monitoring the changes of mitochondrial viscosity during cuprotosis via phosphorescence lifetime imaging. The Ir complex probe possessed microsecond lifetimes (up to 1 µs), which could be easily distinguished from cellular autofluorescence to improve the imaging contrast and sensitivity. Benefiting from the long phosphorescence lifetime, excellent viscosity selectivity, and mitochondrial targeting abilities, the Ir complex probe could monitor the increase in the mitochondrial viscosity during cuproptosis (from 46.8 to 68.9 cP) in a quantitative manner. Moreover, through in situ fluorescence imaging, the Ir complex probe successfully monitored the increase in viscosity in zebrafish treated with lipopolysaccharides or elescolomol-Cu2+, which were well-known cuproptosis inducers. We anticipate that this new Ir complex probe will be a useful tool for in-depth understanding of the biological effects of mitochondrial viscosity during cuproptosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Iridio Límite: Animals / Humans Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Iridio Límite: Animals / Humans Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos