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Mammalian adaptation risk in HPAI H5N8: a comprehensive model bridging experimental data with mathematical insights.
Chokkakula, Santosh; Oh, Sol; Choi, Won-Suk; Kim, Chang Il; Jeong, Ju Hwan; Kim, Beom Kyu; Park, Ji-Hyun; Min, Seong Cheol; Kim, Eung-Gook; Baek, Yun Hee; Choi, Young Ki; Song, Min-Suk.
Afiliación
  • Chokkakula S; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Oh S; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Choi WS; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Kim CI; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Jeong JH; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Kim BK; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Park JH; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Min SC; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Kim EG; Department of Biochemistry, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Baek YH; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
  • Choi YK; Center for Study of Emerging and Re-emerging Viruses, Korea Virus Research Institute, Institute for Basic Science (IBS), Daejeon, Republic of Korea.
  • Song MS; Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Emerg Microbes Infect ; 13(1): 2339949, 2024 Dec.
Article en En | MEDLINE | ID: mdl-38572657
ABSTRACT
Understanding the mammalian pathogenesis and interspecies transmission of HPAI H5N8 virus hinges on mapping its adaptive markers. We used deep sequencing to track these markers over five passages in murine lung tissue. Subsequently, we evaluated the growth, selection, and RNA load of eight recombinant viruses with mammalian adaptive markers. By leveraging an integrated non-linear regression model, we quantitatively determined the influence of these markers on growth, adaptation, and RNA expression in mammalian hosts. Furthermore, our findings revealed that the interplay of these markers can lead to synergistic, additive, or antagonistic effects when combined. The elucidation distance method then transformed these results into distinct values, facilitating the derivation of a risk score for each marker. In vivo tests affirmed the accuracy of scores. As more mutations were incorporated, the overall risk score of virus heightened, and the optimal interplay between markers became essential for risk augmentation. Our study provides a robust model to assess risk from adaptive markers of HPAI H5N8, guiding strategies against future influenza threats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gripe Humana / Subtipo H5N8 del Virus de la Influenza A / Gripe Aviar Límite: Animals / Humans Idioma: En Revista: Emerg Microbes Infect Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gripe Humana / Subtipo H5N8 del Virus de la Influenza A / Gripe Aviar Límite: Animals / Humans Idioma: En Revista: Emerg Microbes Infect Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos