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A critical role of Ca2+/calmodulin-dependent protein kinase II in coupling between evening and morning circadian oscillators in the suprachiasmatic nucleus.
Yoshikawa, Tomoko; Honma, Ken-Ichi; Shigeyoshi, Yasufumi; Yamagata, Yoko; Honma, Sato.
Afiliación
  • Yoshikawa T; Photonic Bioimaging Section, Research Center for Cooperative Project, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
  • Honma KI; Department of Chronomedicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
  • Shigeyoshi Y; Department of Anatomy and Neurobiology, Kindai University Faculty of Medicine, Osakasayama, Japan.
  • Yamagata Y; Organization for International Education and Exchange, University of Toyama, Toyama, Japan.
  • Honma S; Department of Chronomedicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Eur J Neurosci ; 60(2): 3828-3842, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38571281
ABSTRACT
Ca2+/calmodulin-dependent protein kinase IIα (CaMKIIα) is widely expressed in the brain and is involved in various functions, including memory formation, mood and sleep. We previously reported that CaMKIIα is involved in the circadian molecular clock. Mice lacking functional CaMKIIα (K42R mice) exhibited a gradual increase in activity time (α decompression) of running-wheel (RW) activity due to a lengthened circadian period (τ) of activity offset under constant darkness (DD). In the present study, to investigate the functional roles of CaMKIIα in behavioural rhythms, we measured RW and general movements simultaneously under prolonged DD. Tau became longer as the relative intensity of behaviour activity within an activity time shifted from activity onset towards activity offset. In some K42R mice, α was gradually expanded with a marked reduction of RW activity, while general movements persisted without noticeable decline, which was followed by an abrupt shortening of α (α compression) with differential phase shifts of the activity onset and offset and recovery of RW activity. These results suggest that an internal coupling between the oscillators controlling activity onset and offset is bidirectional but with different strengths. The α compression occurred recurrently in 38% of K42R mice examined with an average interval of 37 days in association with attenuation of RW activity but never in the wild-type (WT) mice. Consistent with behavioural rhythms, the circadian period of the PER2LUC rhythm in the cultured suprachiasmatic nucleus (SCN) slice was significantly longer in K42R than in WT. These findings are best interpreted by assuming that a loss of functional CaMKIIα attenuates the coupling between the onset and offset oscillators.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Supraquiasmático / Ritmo Circadiano / Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina Límite: Animals Idioma: En Revista: Eur J Neurosci Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Supraquiasmático / Ritmo Circadiano / Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina Límite: Animals Idioma: En Revista: Eur J Neurosci Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Francia