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Iduronate sulfatase from human placenta.
Biochim Biophys Acta ; 839(3): 258-61, 1985 May 08.
Article en En | MEDLINE | ID: mdl-3857078
The major enzyme component of iduronate sulfatase from human placenta was purified 30 000-fold by a five-step procedure. Sucrose gradient centrifugation of the native enzyme gave a molecular weight estimate of 80 000 +/- 10 000. Electrophoresis in sodium dodecyl sulfate of the enzyme reduced with mercaptoethanol showed a protein band of Mr 82 000. We suggest that the enzyme is composed of a single polypeptide chain of Mr 80 000-90 000.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Sulfatasas / Iduronato Sulfatasa Límite: Female / Humans / Pregnancy Idioma: En Revista: Biochim Biophys Acta Año: 1985 Tipo del documento: Article Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Sulfatasas / Iduronato Sulfatasa Límite: Female / Humans / Pregnancy Idioma: En Revista: Biochim Biophys Acta Año: 1985 Tipo del documento: Article Pais de publicación: Países Bajos