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Applications of Tissue Clearing in Central and Peripheral Nerves.
He, Cheng; Yuan, Ye; Gong, Chuanhui; Wang, Xueying; Lyu, Guangming.
Afiliación
  • He C; Department of Anatomy, Medical School of Nantong University, Nantong, China.
  • Yuan Y; Department of Anatomy, Medical School of Nantong University, Nantong, China.
  • Gong C; Department of Anatomy, Medical School of Nantong University, Nantong, China.
  • Wang X; Medical School of Nantong University, Nantong, China.
  • Lyu G; Department of Anatomy, Medical School of Nantong University, Nantong, China; Department of Anatomy, Institute of Neurobiology, Jiangsu Key Laboratory of Neuroregeneration, Medical School of Nantong University, Nantong, China. Electronic address: gmlu@ntu.edu.cn.
Neuroscience ; 546: 104-117, 2024 May 14.
Article en En | MEDLINE | ID: mdl-38570062
ABSTRACT
The techniques of tissue clearing have been proposed and applied in anatomical and biomedical research since the 19th century. As we all know, the original study of the nervous system relied on serial ultrathin sections and stereoscopic techniques. The 3D visualization of the nervous system was established by software splicing and reconstruction. With the development of science and technology, microscope equipment had constantly been upgraded. Despite the great progress that has been made in this field, the workload is too complex, and it needs high technical requirements. Abundant mistakes due to manual sections were inescapable and structural integrity remained questionable. According to the classification of tissue transparency methods, we introduced the latest application of transparency methods in central and peripheral nerve research from optical imaging, molecular markers and data analysis. This review summarizes the application of transparent technology in neural pathways. We hope to provide some inspiration for the continuous optimization of tissue clearing methods.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Periféricos Límite: Animals / Humans Idioma: En Revista: Neuroscience Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Periféricos Límite: Animals / Humans Idioma: En Revista: Neuroscience Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos