Your browser doesn't support javascript.
loading
Fiber-optic immunosensor based on a Fabry-Perot interferometer for single-molecule detection of biomarkers.
Qiu, Haiming; Yao, Yong; Dong, Yongkang; Tian, Jiajun.
Afiliación
  • Qiu H; Department of Electronic and Information Engineering, Harbin Institute of Technology, Shenzhen, 518055, China; Zhengzhou Research Institute of Harbin Institute of Technology, Zhengzhou, 450000, China.
  • Yao Y; Department of Electronic and Information Engineering, Harbin Institute of Technology, Shenzhen, 518055, China.
  • Dong Y; National Key Laboratory of Science and Technology on Tunable Laser, Harbin Institute of Technology, Harbin, 150001, China; Zhengzhou Research Institute of Harbin Institute of Technology, Zhengzhou, 450000, China.
  • Tian J; Department of Electronic and Information Engineering, Harbin Institute of Technology, Shenzhen, 518055, China; Zhengzhou Research Institute of Harbin Institute of Technology, Zhengzhou, 450000, China. Electronic address: tianjiajun@hit.edu.cn.
Biosens Bioelectron ; 255: 116265, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38569251
ABSTRACT
Immunosensors capable of ultralow-concentration and single-molecule detection of biomarkers are garnering attention for the early diagnosis of cancer. Herein, a fiber-optic Fabry-Perot interferometer (FPI)-based immunosensor was used for the first time for single-molecule detection of progastrin-releasing peptide (ProGRP). The cascaded FPI structure of the immunosensor introduces a high-order harmonic Vernier effect (HVE). A piece of a side-polished D-shaped hollow-core photonic crystal fiber (HCPCF) was used as a sensing FPI, on which the biomarker was deposited to detect ProGRP. Compared with traditional FPIs with open-cavity structures, this structure provided a larger contact area and improved the sensitivity of the immunosensor. The polished side surface of the D-shaped HCPCF was modified using a gold nanoparticle-graphene oxide (AuNP@GO) nanointerface to enhance refractive index (RI) modulation via antigen-antibody binding and achieve selective energy enhancement of the binding site. The antigen binding changes the RI of the D-shaped HCPCF and the effective RI of the transmitted light in the sensing FPI, thereby changing the spectrum of the immunosensor. Experimental results showed that the high-order HVE and AuNP@GO nanointerface considerably improved the immunosensor sensitivity, exhibiting a liquid RI sensitivity of 583,000 nm/RIU. After functionalization with an anti-ProGRP antibody, the limit of detection of the immunosensor for ProGRP reached 17.1 ag/mL; moreover, the immunosensor could perform detection at the single-molecule level. The proposed novel immunosensor overcomes the sensitivity limitations of optical devices and achieves single-molecule detection of a protein.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanopartículas del Metal Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanopartículas del Metal Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido