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Peste des petits ruminants virus infection induces endoplasmic reticulum stress and apoptosis via IRE1-XBP1 and IRE1-JNK signaling pathways.
Yuan, Shuyi; Liu, Yanfen; Mu, Yun; Kuang, Yongshen; Chen, Shaohong; Zhao, Yun-Tao; Liu, You.
Afiliación
  • Yuan S; College of Coastal Agricultural Science of Guangdong Ocean University, Zhanjiang 524088, China.
  • Liu Y; College of Coastal Agricultural Science of Guangdong Ocean University, Zhanjiang 524088, China.
  • Mu Y; College of Coastal Agricultural Science of Guangdong Ocean University, Zhanjiang 524088, China.
  • Kuang Y; College of Coastal Agricultural Science of Guangdong Ocean University, Zhanjiang 524088, China.
  • Chen S; College of Food Science and Technology of Guangdong Ocean University, Zhanjiang 524088, China.
  • Zhao YT; College of Food Science and Technology of Guangdong Ocean University, Zhanjiang 524088, China.
  • Liu Y; College of Food Science and Technology of Guangdong Ocean University, Zhanjiang 524088, China. liuy6254282@163.com.
J Vet Sci ; 25(2): e21, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38568823
ABSTRACT

BACKGROUND:

Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial.

OBJECTIVES:

To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication.

METHODS:

The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway.

RESULTS:

The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly down-regulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010.

CONCLUSIONS:

PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Ovejas / Sulfonamidas / Tiofenos / Butilaminas / Enfermedades de las Cabras / Virus de la Peste de los Pequeños Rumiantes / Peste de los Pequeños Rumiantes Límite: Animals Idioma: En Revista: J Vet Sci Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación:
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Ovejas / Sulfonamidas / Tiofenos / Butilaminas / Enfermedades de las Cabras / Virus de la Peste de los Pequeños Rumiantes / Peste de los Pequeños Rumiantes Límite: Animals Idioma: En Revista: J Vet Sci Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: