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Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform.
Kampmann, Marie-Louise; Fleckhaus, Jan; Børsting, Claus; Jurtikova, Helena; Piters, Alice; Papin, Julien; Gauthier, Quentin; Ghemrawi, Mirna; Doutremepuich, Christian; McCord, Bruce; Schneider, Peter M; Drabek, Jiri; Morling, Niels.
Afiliación
  • Kampmann ML; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark.
  • Fleckhaus J; Institute of Legal Medicine, Faculty of Medicine and University Clinic, University of Cologne, Cologne, Germany.
  • Børsting C; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark.
  • Jurtikova H; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and the University Hospital Olomouc, Olomouc, the Czech Republic.
  • Piters A; Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France.
  • Papin J; Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France.
  • Gauthier Q; Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
  • Ghemrawi M; Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
  • Doutremepuich C; Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France.
  • McCord B; Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
  • Schneider PM; Institute of Legal Medicine, Faculty of Medicine and University Clinic, University of Cologne, Cologne, Germany.
  • Drabek J; Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and the University Hospital Olomouc, Olomouc, the Czech Republic.
  • Morling N; Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark.
Forensic Sci Res ; 9(1): owad055, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38567377
ABSTRACT
Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of ELOVL2, C1orf132, TRIM59, KLF14, and FHL2), and sequencing of the amplicons using the PyroMark Q48 platform. Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed. An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set. Key points Age determination as an intelligence tool during investigations can be a powerful tool in forensic genetics.In this study, five laboratories ran 49 samples and obtained a mean absolute deviation of 3.62 years.Five markers were analyzed on a PyroMark Q48 platform.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Forensic Sci Res Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Forensic Sci Res Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido