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Optimal Treatment Parameters for Ultrasound-Stimulated Microbubbles in Upregulating Proliferation and Stemness of Bone Marrow Mesenchymal Stem Cells.
Chen, Beibei; Zhu, Qiong; Duan, Mao; Li, Qinglong; Wang, Gong; Guan, Xue; Yu, Pu; Xu, Xiaoxun; He, Ying; Xu, Yali.
Afiliación
  • Chen B; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
  • Zhu Q; Department of Ultrasound, Postgraduate Training Basement of Jinzhou Medical University, The PLA Rocket Force Characteristic Medical Center, Beijing, China.
  • Duan M; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
  • Li Q; Department of Ultrasound, 953th Hospital, Shigatse Branch, Xinqiao Hospital, Army Medical University, Shigatse, China.
  • Wang G; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
  • Guan X; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
  • Yu P; Department of Ultrasound, Postgraduate Training Basement of Jinzhou Medical University, The PLA Rocket Force Characteristic Medical Center, Beijing, China.
  • Xu X; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
  • He Y; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
  • Xu Y; Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing, China.
J Ultrasound Med ; 43(7): 1333-1342, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38563453
ABSTRACT

OBJECTIVES:

Ultrasound-targeted microbubble disruption (UTMD) is a widely used technique to improve the differentiation and proliferation capacity of mesenchymal stem cells (MSCs), but the optimal therapeutic parameters for UTMD are unclear. In this study, we aimed to find the appropriate peak negative pressure (PNP), which is a key parameter for enhancing the stemness properties and proliferation of MSCs.

METHODS:

Experiments were performed in UTMD group, ultrasound (US) group under different PNP exposure conditions (0.5, 1.0, and 1.5 MPa), and control group. Apoptosis safety was analyzed by flow cytometry and MSC proliferation was measured at 12, 24, and 36 hours after irradiation by cell counting kit 8. The expression of the stemness genes NANOG, OCT-4, and SOX-2 were determined by enzyme-linked immunosorbent assay (ELISA) or reverse transcription polymerase chain reaction.

RESULTS:

The results showed that the 1.5 MPa UTMD-treated group had the highest proliferation capacity of MSCs at 24 hours. ELISA or quantitative reverse transcription polymerase chain reaction results showed that UTMD treatment of the 1.5 MPa group significantly upregulated the expression of the stemness genes NANOG, SOX-2, and OCT-4.

CONCLUSIONS:

In conclusion, the appropriate peak PNP value of UTMD was 1.5 MPa, and 1.5 MPa-mediated UTMD group obviously promoted MSCs proliferation and maintained stemness by upregulating the expression of stemness genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación hacia Arriba / Microburbujas / Proliferación Celular / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: J Ultrasound Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación hacia Arriba / Microburbujas / Proliferación Celular / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: J Ultrasound Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido