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The Effects of Grape Seed Oligomeric Proanthocyanidin and Nisin on Dental Pulp Stem Cells.
Ballikaya, Elif; Babadag, Sena; San Keskin, Nalan Oya; Çelebi-Saltik, Betül.
Afiliación
  • Ballikaya E; Department of Oral and Dental Health Research, Hacettepe University Graduate School of Health Sciences, Ankara, Turkey.
  • Babadag S; Department of Pediatric Dentistry, Hacettepe University Faculty of Dentistry, Ankara, Turkey.
  • San Keskin NO; Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara, Turkey.
  • Çelebi-Saltik B; Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
Acta Stomatol Croat ; 58(1): 2-17, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38562220
ABSTRACT

Objective:

This study aimed to evaluate the biological effects of "proanthocyanidin" (PA), and "nisin" (Ni), on dental pulp stem cells (DPSCs) and LPS-induced DPSCs as well as their antimicrobial effects against S. aureus and E. coli. Materials and

methods:

After characterization of DPSCs, cytotoxicity of PA and Ni on DPSCs were evaluated using a water-soluble tetrazolium salt (WST-1). The cytokines and chemokines released by DPSCs and the expression levels of IL-6, IL-8, and TNF alpha were detected with human Cytokine Array C5 and enzyme-linked immunosorbent assay (ELISA), respectively. The antibacterial activities of PA and Ni were tested using the drop plate method.

Results:

PA at 75 µg/ml increased cell viability, decreased TNF-α expression of DPSCs, did not show any cytotoxic effects on LPS-induced DPSCs, and also showed a tendency to decrease TNF-α expression. PA at 75 µg/ml exhibited higher expressions of TIMP-2, OPG, IL-7, and IL-8 in LPS-induced DPSCs compared to DPSCs. Ni at 100 µg/ml decreased TNF-α expression in DPSCs with no cytotoxic effects. It provided increased cell viability and a downregulation trend of TNF-α expression in LPS-induced DPSCs. Both Ni and PA provided strong antibacterial effects against S. aureus. Ni at 200µg/ml had strong antibacterial effects against E. coli without affecting negatively the viability of both DPSCs and LPS-induced DPSCs and showed anti-inflammatory activity by decreasing TNF-α expression. PA provided strong antibacterial effects against E. coli at 200 µg/ml but affected DPSCs viability negatively.

Conclusion:

PA and Ni at specific concentrations exhibited immunomodulatory activity on DPSCs and LPS-induced DPSCs without any cytotoxic effects and strong antibacterial effects on S. aureus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Acta Stomatol Croat Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Croacia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Acta Stomatol Croat Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Croacia