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Functional screening of the Arabidopsis 2C protein phosphatases family identifies PP2C15 as a negative regulator of plant immunity by targeting BRI1-associated receptor kinase 1.
Diao, Zhihong; Yang, Rongqian; Wang, Yizhu; Cui, Junmei; Li, Junhao; Wu, Qiqi; Zhang, Yaxin; Yu, Xiaosong; Gong, Benqiang; Huang, Yan; Yu, Guozhi; Yao, Huipeng; Guo, Jinya; Zhang, Huaiyu; Shen, Jinbo; Gust, Andrea A; Cai, Yi.
Afiliación
  • Diao Z; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Yang R; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Wang Y; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Cui J; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Li J; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Wu Q; Chengdu Lusyno Biotechnology Co., Ltd., Chengdu, China.
  • Zhang Y; Chengdu Lusyno Biotechnology Co., Ltd., Chengdu, China.
  • Yu X; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Gong B; Guangdong Provincial Key Laboratory of Plant Resources, State Key Laboratory of Biocontrol, MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Huang Y; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Yu G; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Yao H; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Guo J; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Zhang H; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
  • Shen J; Zhejiang A&F University State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F University, Zhejiang, Hangzhou, China.
  • Gust AA; Department of the Centre for Plant Molecular Biology, Plant Biochemistry, Eberhard Karls University of Tübingen, Tübingen, Germany.
  • Cai Y; Department of Biotechnology and Applied Biology, College of Life Sciences, Sichuan Agricultural University, Ya'an, Sichuan, China.
Mol Plant Pathol ; 25(4): e13447, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38561315
ABSTRACT
Genetic engineering using negative regulators of plant immunity has the potential to provide a huge impetus in agricultural biotechnology to achieve a higher degree of disease resistance without reducing yield. Type 2C protein phosphatases (PP2Cs) represent the largest group of protein phosphatases in plants, with a high potential for negative regulatory functions by blocking the transmission of defence signals through dephosphorylation. Here, we established a PP2C functional protoplast screen using pFRK1luciferase as a reporter and found that 14 of 56 PP2Cs significantly inhibited the immune response induced by flg22. To verify the reliability of the system, a previously reported MAPK3/4/6-interacting protein phosphatase, PP2C5, was used; it was confirmed to be a negative regulator of PAMP-triggered immunity (PTI). We further identified PP2C15 as an interacting partner of BRI1-associated receptor kinase 1 (BAK1), which is the most well-known co-receptor of plasma membrane-localized pattern recognition receptors (PRRs), and a central component of PTI. PP2C15 dephosphorylates BAK1 and negatively regulates BAK1-mediated PTI responses such as MAPK3/4/6 activation, defence gene expression, reactive oxygen species bursts, stomatal immunity, callose deposition, and pathogen resistance. Although plant growth and 1000-seed weight of pp2c15 mutants were reduced compared to those of wild-type plants, pp2c5 mutants did not show any adverse effects. Thus, our findings strengthen the understanding of the mechanism by which PP2C family members negatively regulate plant immunity at multiple levels and indicate a possible approach to enhance plant resistance by eliminating specific PP2Cs without affecting plant growth and yield.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Mol Plant Pathol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Mol Plant Pathol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido