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Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging.
Harmon, Jennifer N; Chandran, Preeja; Chandrasekaran, Abarajithan; Hyde, Jeffrey E; Hernandez, Gustavo J; Reed, May J; Bruce, Matthew F; Khaing, Zin Z.
Afiliación
  • Harmon JN; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA.
  • Chandran P; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA.
  • Chandrasekaran A; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA.
  • Hyde JE; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA.
  • Hernandez GJ; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA.
  • Reed MJ; Department of Gerontology and Geriatric Medicine, University of Washington, Seattle, WA, USA.
  • Bruce MF; Applied Physics Laboratory, University of Washington, Seattle, WA, USA.
  • Khaing ZZ; Department of Neurological Surgery, University of Washington, 1959 NE Pacific St., Seattle, WA, USA.
bioRxiv ; 2024 Mar 14.
Article en En | MEDLINE | ID: mdl-38559128
ABSTRACT
Normal aging is associated with significant deleterious cerebrovascular changes; these have been implicated in disease pathogenesis and increased susceptibility to ischemic injury. While these changes are well documented in the brain, few studies have been conducted in the spinal cord. Here, we utilize specialized contrast-enhanced ultrasound (CEUS) imaging to investigate age-related changes in cervical spinal vascular anatomy and hemodynamics in male Fisher 344 rats, a common strain in aging research. Aged rats (24-26 mo., N=6) exhibited significant tortuosity in the anterior spinal artery and elevated vascular resistance compared to adults (4-6 mo., N=6; tortuosity index 2.20±0.15 vs 4.74±0.45, p<0.05). Baseline blood volume was lower in both larger vessels and the microcirculation in the aged cohort, specifically in white matter (4.44e14±1.37e13 vs 3.66e14±2.64e13 CEUS bolus AUC, p<0.05). To elucidate functional differences, animals were exposed to a hypoxia challenge; whereas adult rats exhibited significant functional hyperemia in both gray and white matter (GM 1.13±0.10-fold change from normoxia, p<0.05; WM 1.16±0.13, p<0.05), aged rats showed no response. Immunohistochemistry revealed reduced pericyte coverage and activated microglia behavior in aged rats, which may partially explain the lack of vascular response. This study provides the first in vivo description of age-related hemodynamic differences in the cervical spinal cord.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos