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What is slough? Defining the proteomic and microbial composition of slough and its implications for wound healing.
Townsend, Elizabeth C; Cheong, J Z Alex; Radzietza, Michael; Fritz, Blaine; Malone, Matthew; Bjarnsholt, Thomas; Ousey, Karen; Swanson, Terry; Schultz, Gregory; Gibson, Angela L F; Kalan, Lindsay R.
Afiliación
  • Townsend EC; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
  • Cheong JZA; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Radzietza M; Medical Scientist Training Program, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
  • Fritz B; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
  • Malone M; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
  • Bjarnsholt T; Infectious Diseases and Microbiology, Western Sydney University, Sydney, Australia.
  • Ousey K; Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Swanson T; Infectious Diseases and Microbiology, Western Sydney University, Sydney, Australia.
  • Schultz G; Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Gibson ALF; Department of Clinical Microbiology, Copenhagen University Hospital, Copenhagen, Denmark.
  • Kalan LR; International Wound Infection Institute, London, UK.
Wound Repair Regen ; 2024 Apr 01.
Article en En | MEDLINE | ID: mdl-38558438
ABSTRACT
Slough is a well-known feature of non-healing wounds. This pilot study aims to determine the proteomic and microbiologic components of slough as well as interrogate the associations between wound slough components and wound healing. Ten subjects with slow-to-heal wounds and visible slough were enrolled. Aetiologies included venous stasis ulcers, post-surgical site infections and pressure ulcers. Patient co-morbidities and wound healing outcome at 3-months post-sample collection was recorded. Debrided slough was analysed microscopically, through untargeted proteomics, and high-throughput bacterial 16S-ribosomal gene sequencing. Microscopic imaging revealed wound slough to be amorphous in structure and highly variable. 16S-profiling found slough microbial communities to associate with wound aetiology and location on the body. Across all subjects, slough largely consisted of proteins involved in skin structure and formation, blood-clot formation and immune processes. To predict variables associated with wound healing, protein, microbial and clinical datasets were integrated into a supervised discriminant analysis. This analysis revealed that healing wounds were enriched for proteins involved in skin barrier development and negative regulation of immune responses. While wounds that deteriorated over time started off with a higher baseline Bates-Jensen Wound Assessment Score and were enriched for anaerobic bacterial taxa and chronic inflammatory proteins. To our knowledge, this is the first study to integrate clinical, microbiome, and proteomic data to systematically characterise wound slough and integrate it into a single assessment to predict wound healing outcome. Collectively, our findings underscore how slough components can help identify wounds at risk of continued impaired healing and serves as an underutilised biomarker.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Wound Repair Regen Asunto de la revista: DERMATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Wound Repair Regen Asunto de la revista: DERMATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos