Critical role of cyclic electron transport around photosystem I in the maintenance of photosystem I activity.

Kobayashi, Ryouhei; Yamamoto, Hiroshi; Ishibashi, Kota; Shikanai, Toshiharu

Kobayashi, Ryouhei; Yamamoto, Hiroshi; Ishibashi, Kota; Shikanai, Toshiharu.

Afiliación

Kobayashi R; Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.
Yamamoto H; Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.
Ishibashi K; Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.
Shikanai T; Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.

Plant J ; 118(6): 2141-2153, 2024 Jun.

Article en En | MEDLINE | ID: mdl-38558422

ABSTRACT

ABSTRACT

In angiosperms, cyclic electron transport around photosystem I (PSI) is mediated by two pathways that depend on the PROTON GRADIENT REGULATION 5 (PGR5) protein and the chloroplast NADH dehydrogenase-like (NDH) complex, respectively. In the Arabidopsis double mutants defective in both pathways, plant growth and photosynthesis are impaired. The pgr5-1 mutant used in the original study is a missense allele and accumulates low levels of PGR5 protein. In this study, we generated two knockout (KO) alleles, designated as pgr5-5 and pgr5-6, using the CRISPR-Cas9 technology. Although both KO alleles showed a severe reduction in P700 similar to the pgr5-1 allele, NPQ induction was less severely impaired in the KO alleles than in the pgr5-1 allele. In the pgr5-1 allele, the second mutation affecting NPQ size was mapped to ~21 cM south of the pgr5-1 locus. Overexpression of the pgr5-1 allele, encoding the glycine130-to-serine change, complemented the pgr5-5 phenotype, suggesting that the pgr5-1 mutation destabilizes PGR5 but that the mutant protein retains partial functionality. Using two KO alleles, we created the double mutants with two chlororespiratory reduction (crr) mutants defective in the NDH complex. The growth of the double mutants was notably impaired. In the double mutant seedlings that survived on the medium containing sucrose, PSI activity evaluated by the P700 oxidation was severely impaired, whereas PSII activity was only mildly impaired. Cyclic electron transport around PSI is required to maintain PSI activity.

Asunto(s)

Proteínas de Arabidopsis; Arabidopsis; Fotosíntesis; Proteínas del Complejo del Centro de Reacción Fotosintética; Complejo de Proteína del Fotosistema I; Complejo de Proteína del Fotosistema I/metabolismo; Complejo de Proteína del Fotosistema I/genética; Transporte de Electrón; Arabidopsis/genética; Arabidopsis/metabolismo; Proteínas de Arabidopsis/metabolismo; Proteínas de Arabidopsis/genética; Proteínas del Complejo del Centro de Reacción Fotosintética/metabolismo; Proteínas del Complejo del Centro de Reacción Fotosintética/genética; Cloroplastos/metabolismo; Mutación

Palabras clave

Arabidopsis thaliana; NDH; PGR5; cyclic electron transport; photosystem I

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotosíntesis / Arabidopsis / Proteínas de Arabidopsis / Proteínas del Complejo del Centro de Reacción Fotosintética / Complejo de Proteína del Fotosistema I Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

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