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Availability of individual proteins for quantitative analysis in postmortem brains preserved in two different brain banks.
Nagaoka, Atsuko; Hino, Mizuki; Izumi, Ryuta; Shishido, Risa; Ishibashi, Miki; Hatano, Masataka; Sainouchi, Makoto; Kakita, Akiyoshi; Tomita, Hiroaki; Kunii, Yasuto.
Afiliación
  • Nagaoka A; Department of Psychiatry, Tohoku University Hospital, Sendai, Japan.
  • Hino M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
  • Izumi R; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
  • Shishido R; Department of Disaster Psychiatry, International Research Institute of Disaster Science, Tohoku University, Sendai, Japan.
  • Ishibashi M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
  • Hatano M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
  • Sainouchi M; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
  • Kakita A; Department of Neuropsychiatry, School of Medicine, Fukushima Medical University, Fukushima, Japan.
  • Tomita H; Department of Pathology, Brain Research Institute, Niigata University, Niigata, Japan.
  • Kunii Y; Department of Pathology, Brain Research Institute, Niigata University, Niigata, Japan.
Neuropsychopharmacol Rep ; 44(2): 399-409, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38558385
ABSTRACT

AIM:

Postmortem brain research is necessary for elucidating the pathology of schizophrenia; an increasing number of studies require a combination of suitable tissue samples preserved at multiple brain banks. In this study, we examined whether a comparative study of protein expression levels can be conducted using postmortem brain samples preserved in different facilities.

METHODS:

We compared the demographic factors of postmortem brain samples preserved in two institutions and measured and compared the expression levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glial fibrillary acidic protein (GFAP) in the prefrontal cortex and superior temporal gyrus. GAPDH is generally used as a loading control for western blotting, and GFAP is considered as an astrocyte marker in the brain.

RESULTS:

We found significant differences between the two institutions in postmortem interval, age at death, and preservation time. To reduce the effects of these differences on our measurements, the parameters were set as covariates in our analyses of covariance. Subsequently, no differences in GAPDH and GFAP expression were found between institutions.

CONCLUSIONS:

When studies are conducted using brain samples preserved in different brain banks, differences in demographic factors should be carefully considered and taken into account by statistical methods to minimize their impact as much as possible. Since there was no significant difference in the protein expression levels of GAPDH and GFAP in either region between the two institutions that preserved the postmortem brains, we concluded that it is possible to perform protein quantitative analysis assuming that there is no effect of difference between two institutions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bancos de Tejidos / Proteína Ácida Fibrilar de la Glía Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Neuropsychopharmacol Rep Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bancos de Tejidos / Proteína Ácida Fibrilar de la Glía Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Neuropsychopharmacol Rep Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos