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Melatonin-mediated corrective changes in gut microbiota of experimentally chronodisrupted C57BL/6J mice.
Vohra, Aliasgar; Karnik, Rhydham; Desai, Mansi; Vyas, Hitarthi; Kulshrestha, Shruti; Upadhyay, Kapil Kumar; Koringa, Prakash; Devkar, Ranjitsinh.
Afiliación
  • Vohra A; Division of Chronobiology and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India.
  • Karnik R; Department of Neurology, School of Medicine, Washington University, St. Louis, Missouri, USA.
  • Desai M; Division of Chronobiology and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India.
  • Vyas H; Dr Vikram Sarabhai Institute of Cell and Molecular Biology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India.
  • Kulshrestha S; Department of Animal Biotechnology, College of Veterinary Sciences & A.H., Anand Agricultural University, Anand, India.
  • Upadhyay KK; Department of Internal Medicine, Division of Gastroenterology & Hepatology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
  • Koringa P; Division of Chronobiology and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India.
  • Devkar R; Department of Internal Medicine, Division of Gastroenterology & Hepatology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Chronobiol Int ; 41(4): 548-560, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38557404
ABSTRACT
Chronic consumption of a high-calorie diet coupled with an altered sleep-wake cycle causes disruption of circadian clock that can impact the gut microbiome leading to metabolic syndrome and associated diseases. Herein, we investigate the effects of a high fat high fructose diet (H) alone or in combination with photoperiodic shifts induced chronodisruption (CD) on gut microbiota of C57BL/6J male mice. Further, the merits of daily evening intraperitoneal administration of melatonin in restoring gut microbiota are studied herein. Experimental groups viz. H, CD and HCD mice recorded higher levels of serum pro-inflammatory cytokines (TNF-α and IL-6) and lower levels of the anti-inflammatory cytokine, IL-10. These findings correlate with a concomitant increase in the transcripts of TLR4, TNF-α, and IL-6 in small intestine of the said groups. A decrement in mRNA levels of Ocln, ZO-1 and Vdr in these groups implied towards an altered gut permeability. These results were in agreement with the observed decrement in percentage abundance of total gut microflora and Firmicutes Bacteroidetes (F/B) ratio. Melatonin administration accounted for lower-level inflammation (serum and gut) along with an improvement in gut permeability markers. The total abundance of gut microflora and F/B ratio showed an improvement in all the melatonin-treated groups and the same is the highlight of this study. Taken together, our study is the first to report perturbations in gut microbiota resulting due to a combination of photoperiodic shifts induced CD and a high fat high calorie diet-induced lifestyle disorder. Further, melatonin-mediated rejuvenation of gut microbiome provides prima facie evidence of its role in improving gut dysbiosis that needs a detailed scrutiny.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ritmo Circadiano / Dieta Alta en Grasa / Microbioma Gastrointestinal / Melatonina / Ratones Endogámicos C57BL Límite: Animals Idioma: En Revista: Chronobiol Int Asunto de la revista: FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ritmo Circadiano / Dieta Alta en Grasa / Microbioma Gastrointestinal / Melatonina / Ratones Endogámicos C57BL Límite: Animals Idioma: En Revista: Chronobiol Int Asunto de la revista: FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido