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Paper electrochemical immunosensor for the rapid screening of Galectin-3 patients with heart failure.
Nihal, Serena; Sarfo, Daniel; Zhang, Xi; Tesfamichael, Tuquabo; Karunathilaka, Nuwan; Punyadeera, Chamindie; Izake, Emad L.
Afiliación
  • Nihal S; School of Chemistry and Physics, Faculty of Science, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia.
  • Sarfo D; School of Chemistry and Physics, Faculty of Science, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia; Nuclear and Analytical Chemistry Research Center (NACRC), Ghana Atomic Energy Commission, Ghana.
  • Zhang X; Menzies Health Institute Queensland (MIHQ), Griffith University, Queensland, Australia.
  • Tesfamichael T; School of Chemistry and Physics, Faculty of Science, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia; School of Mechanical, Medical & Process Engineering, Faculty of Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD
  • Karunathilaka N; Menzies Health Institute Queensland (MIHQ), Griffith University, Queensland, Australia.
  • Punyadeera C; Griffith Institute for Drug Discovery (GRIDD), Griffith University, Queensland, Australia; Menzies Health Institute Queensland (MIHQ), Griffith University, Queensland, Australia.
  • Izake EL; School of Chemistry and Physics, Faculty of Science, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia; Centre for Materials Science, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia; Centre for Biomedical Technology,
Talanta ; 274: 126012, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38554482
ABSTRACT
A paper electrochemical immunosensor for the combined binding and quantification of the heart failure (HF) biomarker Galectin-3 has been developed. The simple design of the new sensor is comprised of paper material that is decorated with gold nanostructures, to maximize its electroactive surface area, and functionalized with target-specific recognition molecules to selectively bind the protein from aqueous solutions. The binding of the protein caused the blockage of the electron flow to the sensor electroactive surface, thus causing its oxidation potential to shift and the corresponding current to reduce quantitatively with the increase in the protein concentration within the working range of 0.5ng/mL-8ng/mL (LOQ-0.5 ng/mL). This novel sensor was able to quantify Galectin-3 concentration in saliva samples from HF patients and healthy controls within 20 min with good reproducibility (RSD = 3.64%), without the need for complex sample processing steps. The electrochemical measurements of the patient samples were cross validated by ELISA where the percent agreement between the two methods was found to be 92.7% (RSD = 7.20%). Therefore, the new paper immunosensor sensor has a strong potential for rapid and cost-effective screening of the Galectin 3 biomarker at points of care, thus supporting the timely diagnosis of heart failure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Proteínas Sanguíneas / Técnicas Biosensibles / Galectina 3 / Técnicas Electroquímicas / Insuficiencia Cardíaca Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Proteínas Sanguíneas / Técnicas Biosensibles / Galectina 3 / Técnicas Electroquímicas / Insuficiencia Cardíaca Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos