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Carprofen Attenuates Postoperative Mechanical and Thermal Hypersensitivity after Plantar Incision in Immunodeficient NSG Mice.
Alamaw, Eden D; Casey, Kerriann M; Tien, Krystal; Franco, Benjamin D; Gorman, Gregory; Cotton, Renee M; Nagamine, Claude; Jampachaisri, Katechan; Sharp, Patrick; Pacharinsak, Cholawat; Huss, Monika K.
Afiliación
  • Alamaw ED; Department of Comparative Medicine, Stanford University, Stanford, California;, Email: alamaw@stanford.edu.
  • Casey KM; Department of Comparative Medicine, Stanford University, Stanford, California.
  • Tien K; Department of Comparative Medicine, Stanford University, Stanford, California.
  • Franco BD; Department of Comparative Medicine, Stanford University, Stanford, California.
  • Gorman G; Department of Pharmaceutical, Social and Administrative Sciences, Samford University, Birmingham, Alabama.
  • Cotton RM; Department of Comparative Medicine, Stanford University, Stanford, California.
  • Nagamine C; Department of Comparative Medicine, Stanford University, Stanford, California.
  • Jampachaisri K; Department of Mathematics, Naresuan University, Phitsanulok, Thailand.
  • Sharp P; UC Merced, Merced, California.
  • Pacharinsak C; Department of Comparative Medicine, Stanford University, Stanford, California.
  • Huss MK; Department of Comparative Medicine, Stanford University, Stanford, California.
Comp Med ; 74(2): 105-114, 2024 Apr 01.
Article en En | MEDLINE | ID: mdl-38553034
ABSTRACT
Immunodeficient NSG mice are reported to be less responsive to buprenorphine analgesia. Here, we used NSG mice to compare the efficacy of the commonly used dose of carprofen (5 mg/kg) with 5 and 10 times that dose (25 and 50 mg/kg) for attenuating postoperative mechanical and thermal hypersensitivity following an incisional pain model. Male and female NSG mice (n = 45) were randomly assigned to one of 4 groups and received daily subcutaneous injections for 3 d saline (5 mL/kg), 5 mg/kg carprofen (Carp5), 25 mg/kg carprofen (Carp25), and 50 mg/kg carprofen (Carp50). Mechanical and thermal hypersensitivity were assessed 24 h before and at 4, 24, and 48 h after surgery. Plasma carprofen concentrations were measured in a separate group of mice (n = 56) on days 0 (at 2, 4, 12, and 23 h), 1, and 2 after the first, second, and third doses, respectively. Toxicity was assessed through daily fecal occult blood testing (n = 27) as well as gross and histopathologic evaluation (n = 15). Our results indicated that the saline group showed both mechanical and thermal hypersensitivity throughout the study. Carp5 did not attenuate mechanical or thermal hypersensitivity at any time point. Carp25 attenuated mechanical and thermal (except for the 4-h time point) hypersensitivity. Carp50 attenuated only thermal hypersensitivity at 24 h. Fecal occult blood was detected in 1 of 8 Carp25-treated mice at 48 and 72 h. Histopathologic abnormalities (gastric ulceration, ulcerative enteritis, and renal lesions) were observed in some Carp50-treated mice. Plasma carprofen concentrations were dose and time dependent. Our results indicate that Carp25 attenuated postoperative mechanical and thermal hypersensitivity more effectively than Carp5 or Carp50 in NSG mice with incisional pain. Therefore, we recommend providing carprofen at 25 mg/kg SID for incisional pain procedures using immunodeficient NSG mouse.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dolor Postoperatorio / Carbazoles Límite: Animals Idioma: En Revista: Comp Med Asunto de la revista: MEDICINA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dolor Postoperatorio / Carbazoles Límite: Animals Idioma: En Revista: Comp Med Asunto de la revista: MEDICINA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos