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Colorimetric detection of circulating tumor cells in breast cancer based on ladder-branch hybridization chain reaction and DFs/AuNCs nanozyme.
Ren, Dongxia; Wei, Hua; Li, Na; Fu, Wenda; Huang, Zhijun; Yang, Longfei; Mu, Shijie.
Afiliación
  • Ren D; Department of Transfusion Medicine, Tangdu Hospital, Xi'an, 710032, China.
  • Wei H; Department of Transfusion Medicine, Tangdu Hospital, Xi'an, 710032, China.
  • Li N; Department of Transfusion Medicine, Tangdu Hospital, Xi'an, 710032, China.
  • Fu W; Department of Transfusion Medicine, Tangdu Hospital, Xi'an, 710032, China.
  • Huang Z; Guilin University of Electronic Science and Technology, Guilin, 541004, China.
  • Yang L; Department of Transfusion Medicine, Tangdu Hospital, Xi'an, 710032, China. Electronic address: tdyanglf@fmmu.edu.cn.
  • Mu S; Department of Transfusion Medicine, Tangdu Hospital, Xi'an, 710032, China. Electronic address: musj1963@fmmu.edu.cn.
Talanta ; 274: 125921, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38552481
ABSTRACT
Breast cancer is the most common malignant tumor in women, which accounts for 6.9% of all cancer-related deaths. Early diagnosis is crucial for making the best clinical decision and improving the prognosis of patients. Circulating tumor cells (CTCs) have been regarded as significant tumor biomarkers. Herein, we designed a colorimetric biosensor for breast cancer CTCs quantification based on ladder-branch hybridization chain reaction (HCR) and DNA flowers/gold nanoclusters (DFs/AuNCs) nanozyme. With the assistance of complementary DNA labeled on magnetic beads (MBs), the cleavage products of RNA-cleaving DNAzymes (RCDs) could be rapidly captured, subsequently triggering ladder-branch HCR. In addition, the DFs/AuNCs nanozyme was applied for colorimetric analysis, which further improved the sensitivity for the detection of target CTCs. Benefiting from specific RCDs, ladder-branch HCR and DFs/AuNCs, we achieved a superior detection limit of 3 cells/mL as well as a broad linear range of 10 cells/mL to 104 cells/mL. Conclusively, this colorimetric biosensor achieved sensitively and selectively detection of breast cancer CTCs without the participation of enzymes at room temperature, which might provide new insight into the early detection of breast cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Colorimetría / Nanopartículas del Metal / Oro / Células Neoplásicas Circulantes / Hibridación de Ácido Nucleico Límite: Female / Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Colorimetría / Nanopartículas del Metal / Oro / Células Neoplásicas Circulantes / Hibridación de Ácido Nucleico Límite: Female / Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos