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Extracellular vesicles derived from human foreskin cells (hFS-Exo) accelerate cell migration and angiogenesis through MAPK pathway: an in vitro study.
Sagraç, Derya; Aydin, Safa; Kirbas, Oguz Kaan; Öztürkoglu, Dilek; Sahin, Fikrettin.
Afiliación
  • Sagraç D; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
  • Aydin S; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
  • Kirbas OK; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
  • Öztürkoglu D; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey.
  • Sahin F; Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul, Turkey. fsahin@yeditepe.edu.tr.
Mol Biol Rep ; 51(1): 471, 2024 Mar 29.
Article en En | MEDLINE | ID: mdl-38551706
ABSTRACT

BACKGROUND:

Wound healing is one of the important processes in the body. Attempts to create new drugs are of interest due to the side effects of natural and chemical wound healing compounds. To overcome this obstacle, stem cells have been used as healing agents. However, both difficulties in collection and risks such as rejection and teratoma in the recipient body have limited the use of stem cells, directly. Since the potential content of the stem cells can be transferred to the recipient cells by vesicles, small extracellular vesicles have recently become prominent agents. METHODS AND

RESULTS:

The wound-healing effect of extracellular vesicles derived from foreskin cells was investigated in both keratinocyte and endothelial cells. Migration assay, RT-PCR, Col1a1 ELISA and Western Blot experiments were utilized to reveal healing effect of EVs and its possible molecular pathways. EV-treated groups exhibited more proliferative, invasive, and migrative characteristics. When comparing to the control group, new vessel formation was induced in EV groups. An increase in gene levels of growth factors related to wound healing and change in the mitogen-activated protein kinase (MAPK) signaling pathway proteins in EV-treated groups were determined. Possible molecular mechanisms underlying cell movements were associated with the MAPK pathway. It was found that human foreskin cell EVs (hFS-Exo) may have a potential to heal wounds in a short period of time by triggering the MAPK pathway.

CONCLUSIONS:

hFS-Exo could be a new promising wound healing agent in the future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Quinasas Activadas por Mitógenos / Vesículas Extracelulares Límite: Humans / Male Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Quinasas Activadas por Mitógenos / Vesículas Extracelulares Límite: Humans / Male Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Países Bajos