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Identification of fish specimens of the Tocantins River, Brazil, using DNA barcoding.
Lima, Renato Corrêia; de Lima, Sabrina Rufino; Rocha, Marcelo Salles; Dos Anjos, Hélio Daniel Beltrão; Dantas, Yasmin Catarina Alves; Benites, Ignácio Davi Navegante; Queiroz, Cleonilde da Conceição Silva; Fraga, Elmary da Costa; Batista, Jacqueline da Silva.
Afiliación
  • Lima RC; Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Laboratório Temático de Biologia Molecular (LTBM), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, Brazil.
  • de Lima SR; Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Laboratório Temático de Biologia Molecular (LTBM), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, Brazil.
  • Rocha MS; Coordenação de Ciências Biológicas, Universidade do Estado do Amazonas (UEA), Manaus, Brazil.
  • Dos Anjos HDB; Laboratório de Sistemática e Ecologia de peixes, Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, Brazil.
  • Dantas YCA; Programa de Pós-Graduação em Zoologia, Universidade Federal do Amazonas (UFAM), Manaus, Brazil.
  • Benites IDN; Instituto de Ciências Biológicas (ICB), Curso de Biotecnologia, Universidade Federal do Amazonas (UFAM), Manaus, Brazil.
  • Queiroz CDCS; Centro de Ciências Exatas, Naturais e Tecnológicas (CCENT), Universidade Estadual da Região Tocantina do Maranhão (UEMASUL), Imperatriz, Brazil.
  • Fraga EDC; Departamento de Química e Biologia, Universidade Estadual do Maranhão (UEMA), Caxias, Brazil.
  • Batista JDS; Coordenação de Biodiversidade (COBIO), Laboratório Temático de Biologia Molecular (LTBM), Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, Brazil.
J Fish Biol ; 104(6): 1924-1939, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38551122
ABSTRACT
The fish fauna of the Tocantins River possesses many endemic species; however, it is little studied in molecular terms and is quite threatened by the construction of several hydroelectric dams. Therefore, the objective of this study was to identify the ichthyofauna of the Tocantins River using DNA barcoding. For this, collections were carried out in five points of this river, which resulted in the capture of 725 individuals from which partial sequences of the cytochrome oxidase subunit I (COI) gene were obtained for genetic analysis. A total of 443 haplotypes were recovered with the mean intraspecific K2P genetic distance of 1.82%. Altogether, 138 species were identified based on morphological criteria, which was a quantity that was much lower than that indicated by the four molecular methods (assemble species by automatic partitioning [ASAP], barcode index number [BIN], generalized mixed Yule coalescent (GMYC), and Bayesian Poisson tree processes [bPTP]) through which 152-157 molecular entities were identified. In all, 41 unique BINs were obtained based on the data generated in the BOLDSystems platform. According to the result indicated by ASAP (species delimitation approach considered the most appropriate in the present study), there was an increase of 17 molecular entities (12.32%), when compared to the number of species identified through their morphological criteria, as it can show cryptic diversity, candidates for new species, and misidentifications. There were 21 incongruities indicated between the different identification approaches for species. Therefore, it is suggested that these taxonomic problems be cautiously evaluated by experts to solve such taxonomic issues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complejo IV de Transporte de Electrones / Ríos / Código de Barras del ADN Taxonómico / Peces Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Fish Biol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complejo IV de Transporte de Electrones / Ríos / Código de Barras del ADN Taxonómico / Peces Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Fish Biol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido