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Comparative genetic and pathogenic approaches of Escherichia coli isolated simultaneously from pyometra and urine of bitches.
Lopes, Cassiane Elisabete; Weyh, Tainara Soares; Moni, Camila Azevedo; Dias, Maria Eduarda; Gomes, Tania Aparecida Tardelli; da Costa, Mateus Matiuzzi; Ramos, Rommel Thiago Jucá; Góes-Neto, Aristóteles; de Carvalho Azevedo, Vasco Ariston; Brenig, Bertram; Siqueira, Franciele Maboni.
Afiliación
  • Lopes CE; Laboratory of Veterinary Bacteriology, Veterinary School, Federal University of Rio Grande Sul (UFRGS), Porto Alegre, Brazil.
  • Weyh TS; Programa de Pós-Graduação em Ciências Veterinárias, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
  • Moni CA; Laboratory of Veterinary Bacteriology, Veterinary School, Federal University of Rio Grande Sul (UFRGS), Porto Alegre, Brazil.
  • Dias ME; Laboratory of Veterinary Bacteriology, Veterinary School, Federal University of Rio Grande Sul (UFRGS), Porto Alegre, Brazil.
  • Gomes TAT; Laboratory of Veterinary Bacteriology, Veterinary School, Federal University of Rio Grande Sul (UFRGS), Porto Alegre, Brazil.
  • da Costa MM; Laboratório Experimental de Patogenicidade de Enterobactérias (LEPE), Departamento de Microbiologia, Imunologia e Parasitologia (DMIP), Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
  • Ramos RTJ; Department of Biological Sciences, Federal University of Vale do São Francisco, Petrolina, PE, Brazil.
  • Góes-Neto A; Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
  • de Carvalho Azevedo VA; Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Brenig B; Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Siqueira FM; Department of Molecular Biology of Livestock, Institute of Veterinary Medicine, Georg August University Göttingen, Göttingen, Germany.
Vet Res Commun ; 48(3): 1755-1768, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38546809
ABSTRACT
Escherichia coli (E. coli) are widely related to pyometra and cystitis in dogs, and these infections can occur simultaneously. The goal of this study was to determine genetic and pathogenic insights of 14 E. coli isolated simultaneously from pyometra content and bladder urine of seven bitches. To achieve this, in silico and in vitro comparative analyses were conducted. Whole-genome comparisons demonstrated that E. coli isolated from pyometra and urine of the same animal were predominantly genetic extraintestinal E. coli clones belonging to the same Sequence Type and phylogroup. The E. coli clones identified in this study included ST372, ST457, ST12, ST127, ST646, and ST961. Five isolates (35.7%) belonged to the ST12 complex. Except for two E. coli, all other isolates belonged to the B2 Clermont phylogroup. Interestingly, some genomes of E. coli from urine carried more virulence genes than those E. coli from pyometra. Both pyometra and urine E. coli isolates demonstrated a strong affinity for adhering to HeLa and T24 cells, with a low affinity for invading them. However, certain isolates from urine exhibited a greater tendency to adhere to T24 cells in qualitative and quantitative assays compared to isolates from pyometra. In conclusion, this study revealed the high genomic similarity between pyometra and urine E. coli isolates, as well as the virulent capacity of both to colonize endometrial and urothelial cells. The findings of this study underscore the importance of concurrently managing both infections clinically and could potentially contribute to future resources for the prevention of cystitis and pyometra.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Perros / Escherichia coli / Infecciones por Escherichia coli / Piómetra Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Perros / Escherichia coli / Infecciones por Escherichia coli / Piómetra Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza