Your browser doesn't support javascript.
loading
The Effect of HMGB1 and HMGB2 on Transcriptional Regulation Differs in Neuroendocrine and Adenocarcinoma Models of Prostate Cancer.
Salamini-Montemurri, Martín; Vizoso-Vázquez, Ángel; Barreiro-Alonso, Aida; Lorenzo-Catoira, Lidia; Rodríguez-Belmonte, Esther; Cerdán, María-Esperanza; Lamas-Maceiras, Mónica.
Afiliación
  • Salamini-Montemurri M; Centro Interdisciplinar de Química e Bioloxía (CICA), Campus de Elviña, Universidade da Coruña, As Carballeiras, s/n, 15071 A Coruña, Spain.
  • Vizoso-Vázquez Á; Facultade de Ciencias, Campus de A Zapateira, Universidade da Coruña, A Fraga, s/n, 15071 A Coruña, Spain.
  • Barreiro-Alonso A; Instituto de Investigación Biomédica de A Coruña (INIBIC), As Xubias de Arriba 84, 15006 A Coruña, Spain.
  • Lorenzo-Catoira L; Centro Interdisciplinar de Química e Bioloxía (CICA), Campus de Elviña, Universidade da Coruña, As Carballeiras, s/n, 15071 A Coruña, Spain.
  • Rodríguez-Belmonte E; Facultade de Ciencias, Campus de A Zapateira, Universidade da Coruña, A Fraga, s/n, 15071 A Coruña, Spain.
  • Cerdán ME; Instituto de Investigación Biomédica de A Coruña (INIBIC), As Xubias de Arriba 84, 15006 A Coruña, Spain.
  • Lamas-Maceiras M; Centro Interdisciplinar de Química e Bioloxía (CICA), Campus de Elviña, Universidade da Coruña, As Carballeiras, s/n, 15071 A Coruña, Spain.
Int J Mol Sci ; 25(6)2024 Mar 07.
Article en En | MEDLINE | ID: mdl-38542079
ABSTRACT
Human high-mobility group-B (HMGB) proteins regulate gene expression in prostate cancer (PCa), a leading cause of oncological death in men. Their role in aggressive PCa cancers, which do not respond to hormonal treatment, was analyzed. The effects of HMGB1 and HMGB2 silencing upon the expression of genes previously related to PCa were studied in the PCa cell line PC-3 (selected as a small cell neuroendocrine carcinoma, SCNC, PCa model not responding to hormonal treatment). A total of 72% of genes analyzed, using pre-designed primer panels, were affected. HMGB1 behaved mostly as a repressor, but HMGB2 as an activator. Changes in SERPINE1, CDK1, ZWINT, and FN1 expression were validated using qRT-PCR after HMGB1 silencing or overexpression in PC-3 and LNCaP (selected as an adenocarcinoma model of PCa responding to hormonal treatment) cell lines. Similarly, the regulatory role of HMGB2 upon SERPINE1, ZWINT, FN1, IGFPB3, and TYMS expression was validated, finding differences between cell lines. The correlation between the expression of HMGB1, HMGB2, and their targets was analyzed in PCa patient samples and also in PCa subgroups, classified as neuroendocrine positive or negative, in public databases. These results allow a better understanding of the role of HMGB proteins in PCa and contribute to find specific biomarkers for aggressive PCa.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Adenocarcinoma / Proteína HMGB1 Límite: Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Adenocarcinoma / Proteína HMGB1 Límite: Humans / Male Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza