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Identification of Fusarium oxysporum Causing Leaf Blight on Dendrobium chrysotoxum in Yunnan Province, China.
Yang, Jun; Ahmed, Waqar; Zhang, Jinhao; Gao, Shunyu; Wang, Zhenji; Yang, Haiyan; Bai, Xuehui; Luo, Kai; Xu, Chengdong; Ji, Guanghai.
Afiliación
  • Yang J; College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, China.
  • Ahmed W; State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, China.
  • Zhang J; State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, China.
  • Gao S; State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, China.
  • Wang Z; College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, China.
  • Yang H; College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, China.
  • Bai X; College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, China.
  • Luo K; Dehong Insitute of Tropical Agriculture in Yunnan Province, Ruili 678600, China.
  • Xu C; Dehong Insitute of Tropical Agriculture in Yunnan Province, Ruili 678600, China.
  • Ji G; College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, China.
Life (Basel) ; 14(3)2024 Feb 20.
Article en En | MEDLINE | ID: mdl-38541611
ABSTRACT
Leaf-blight disease caused by the Fusarium oxysporum is an emerging problem in Dendrobium chrysotoxum production in China. Symptoms of leaf blight were observed on seedlings of D. chrysotoxum cultivated in a nursery in Ruili City, Yunnan Province, China. In this study, we isolated the Fusarium sp. associated with leaf-blight disease of D. chrysotoxum from the diseased seedlings. A pathogenicity test was performed to fulfill Koch's postulates to confirm the pathogenicity of isolated strains and identified using morphological and molecular techniques. The results revealed that all four isolated Fusarium sp. isolates (DHRL-01~04) produced typical blight symptoms followed by marginal necrosis of leaves on the D. chrysotoxum plants. On the PDA medium, the fungal colony appeared as a white to purplish color with cottony mycelium growth. Microconidia are oval-shaped, whereas macroconidia are sickle-shaped, tapering at both ends with 2-4 septations. The phylogenetic trees were construed based on internal transcribed spacer (ITS), translation elongation factor (EF-1α), and RNA polymerase subunit genes RPB1 and RPB2 genes, respectively, and blasted against the NCBI database for species confirmation. Based on the NCBI database's blast results, the isolates showed that more than 99% identify with Fusarium oxysporum. To our knowledge, this is the first comprehensive report on the identification of Fusarium oxysporum as the causal agent of Dendrobium chrysotoxum leaf blight in Yunnan Province, China, based on morphological and molecular characteristics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Life (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Life (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza